Unprecedented Sensitivity in a Probe for Monitoring Cathepsin B: Chemiluminescence Microscopy Cell-Imaging of a Natively Expressed Enzyme

Michal E. Roth-Konforti, Christoph R. Bauer, Doron Shabat

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

Until recently, chemiluminescence cell images could only be obtained using luciferase-activated probes. Moreover, chemiluminescence microscopy cell-imaging has not been demonstrated for natively expressed enzymes like cathepsin B. Herein, we describe the design, synthesis, and evaluation of the first chemiluminescence probe for the detection and imaging of cathepsin B. The probe activation mechanism relies on the release of a dioxetane intermediate, which undergoes chemiexcitation to emit green light with high efficiency under physiological conditions. Using the probe, we obtained clear images of cancerous leukemia and colon cells. This is the first demonstration of chemiluminescence cell images obtained by a probe for a natively expressed endogenous enzyme. We anticipate that the concept presented in this study will be broadly used to develop analogous probes for other important proteases relevant to biomolecular processes.

שפה מקוריתאנגלית
עמודים (מ-עד)15633-15638
מספר עמודים6
כתב עתAngewandte Chemie - International Edition
כרך56
מספר גיליון49
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 4 דצמ׳ 2017

ASJC Scopus subject areas

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