Tumor tissue explant culture of patient-derived xenograft as potential prioritization tool for targeted therapy

Susmita Ghosh, Manu Prasad, Kiran Kundu, Limor Cohen, Ksenia M. Yegodayev, Jonathan Zorea, Ben Zion Joshua, Batel Lasry, Orr Dimitstein, Anat Bahat-Dinur, Aviram Mizrachi, Vladimir Lazar, Moshe Elkabets, Angel Porgador

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

Despite of remarkable progress made in the head and neck cancer (HNC) therapy, the survival rate of this metastatic disease remain low. Tailoring the appropriate therapy to patients is a major challenge and highlights the unmet need to have a good preclinical model that will predict clinical response. Hence, we developed an accurate and time efficient drug screening method of tumor ex vivo analysis (TEVA) system, which can predict patient-specific drug responses. In this study, we generated six patient derived xenografts (PDXs) which were utilized for TEVA. Briefly, PDXs were cut into 2 × 2 × 2 mm3 explants and treated with clinically relevant drugs for 24 h. Tumor cell proliferation and death were evaluated by immunohistochemistry and TEVA score was calculated. Ex vivo and in vivo drug efficacy studies were performed on four PDXs and three drugs side-by-side to explore correlation between TEVA and PDX treatment in vivo. Efficacy of drug combinations was also ventured. Optimization of the culture timings dictated 24 h to be the time frame to detect drug responses and drug penetrates 2 × 2 × 2 mm3 explants as signaling pathways were significantly altered. Tumor responses to drugs in TEVA, significantly corresponds with the drug efficacy in mice. Overall, this low cost, robust, relatively simple and efficient 3D tissue-based method, employing material from one PDX, can bypass the necessity of drug validation in immune-incompetent PDX-bearing mice. Our data provides a potential rationale for utilizing TEVA to predict tumor response to targeted and chemo therapies when multiple targets are proposed.

שפה מקוריתאנגלית אמריקאית
מספר המאמר17
כתב עתFrontiers in Oncology
כרך9
מספר גיליוןJAN
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 1 ינו׳ 2019

ASJC Scopus subject areas

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טביעת אצבע

להלן מוצגים תחומי המחקר של הפרסום 'Tumor tissue explant culture of patient-derived xenograft as potential prioritization tool for targeted therapy'. יחד הם יוצרים טביעת אצבע ייחודית.

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