There are four dynamically and functionally distinct populations of E-cadherin in cell junctions

Zahra Erami, Paul Timpson, Wu Yao, Ronen Zaidel-Bar, Kurt I. Anderson

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

E-cadherin is a trans-membrane tumor suppressor responsible for epithelial cell adhesion. E-cadherin forms adhesive clusters through combined extra-cellular cis- and trans-interactions and intracellular interaction with the actin cytoskeleton. Here we identify four populations of E-cadherin within cell junctions based on the molecular interactions which determine their mobility and adhesive properties. Adhesive and non-adhesive populations of E-cadherin each consist of mobile and immobile fractions. Up to half of the E-cadherin immobilized in cell junctions is non-adhesive. Incorporation of E-cadherin into functional adhesions require all three adhesive interactions, with deletion of any one resulting in loss of effective cell-cell adhesion. Interestingly, the only interaction which could independently slow the diffusion of E-cadherin was the tail-mediated intra-cellular interaction. The adhesive and non-adhesive mobile fractions of E-cadherin can be distinguished by their sensitivity to chemical cross-linking with adhesive clusters. Our data define the size, mobility, and adhesive properties of four distinct populations of E-cadherin within cell junctions, and support association with the actin cytoskeleton as the first step in adhesion formation.

שפה מקוריתאנגלית
עמודים (מ-עד)1481-1489
מספר עמודים9
כתב עתBiology Open
כרך4
מספר גיליון11
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 2015
פורסם באופן חיצוניכן

ASJC Scopus subject areas

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  • ???subjectarea.asjc.1100.1100???

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