The regulatory network of Vibrio parahaemolyticus type VI secretion system 1

Rotem Ben-Yaakov, Dor Salomon

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים


Type VI secretion systems (T6SSs) are widespread, tightly regulated, protein delivery apparatuses used by Gram-negative bacteria to outcompete their neighbours. The pathogen, Vibrio parahaemolyticus, encodes two T6SSs. These T6SSs are differentially regulated by external conditions. T6SS1, an antibacterial system predominantly found in pathogenic isolates, requires warm marine-like conditions and surface sensing for activation. The regulatory network that governs this activation is not well understood. In this work, we devised a screening methodology that allows us to easily monitor the outcome of bacterial competitions and thus to identify mutants that are defective in T6SS1-mediated bacterial killing. The methodology, termed Bacterial Competition Fluorescence (BaCoF), relies on detection of a fluorescent signal as an indicator of the survival and growth of a T6SS-sensitive, GFP-expressing prey that has been co-cultured with mutants derived from a T6SS+ attacker of interest. Using BaCoF, we screened a random transposon insertion mutant library and identified genes required for V. parahaemolyticus T6SS1 activation, among them TfoY and Tmk. We used epistasis experiments to determine the relationships between the newly identified components and other regulators that were previously described. Thus, we present here a detailed biological understanding of the T6SS1 regulatory network.

שפה מקוריתאנגלית
עמודים (מ-עד)2248-2260
מספר עמודים13
כתב עתEnvironmental Microbiology
מספר גיליון7
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - יולי 2019

ASJC Scopus subject areas

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