The molecular timeline of a reviving bacterial spore

Lior Sinai, Alex Rosenberg, Yoav Smith, Einat Segev, Sigal Ben-Yehuda

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

The bacterial spore can rapidly convert from a dormant to a fully active cell. Here we study this remarkable cellular transition in Bacillus subtilis and reveal the identity of the newly synthesized proteins throughout spore revival. Our analysis uncovers a highly ordered developmental program that correlates with the spore morphological changes and reveals thespatial and temporal molecular events fundamental to reconstruct a cell. As opposed to current knowledge, we found that translation takes place during the earliest revival event, termed germination, a process hitherto considered to occur without the need for any macromolecule synthesis. Furthermore, we demonstrate that translation is required for execution of germination and relies on the bona fide translational factors RpmE and Tig. Our study sheds light on the spore revival process and on the vital building blocks underlying cellular awakening, thereby paving the way for designing new antimicrobial agents to eradicate spore-forming pathogens.

שפה מקוריתאנגלית אמריקאית
עמודים (מ-עד)695-707
מספר עמודים13
כתב עתMolecular Cell
כרך57
מספר גיליון4
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 19 פבר׳ 2015

ASJC Scopus subject areas

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