Real-Time Sensing of Enteropathogenic E. coli-Induced Effects on Epithelial Host Cell Height, Cell-Substrate Interactions, and Endocytic Processes by Infrared Surface Plasmon Spectroscopy

Victor Yashunsky, Leorah Kharilker, Efrat Zlotkin-Rivkin, David Rund, Naomi Melamed-Book, Eitan Erez Zahavi, Eran Perlson, Silvana Mercone, Michael Golosovsky, Dan Davidov, Benjamin Aroeti

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

Enteropathogenic Escherichia coli (EPEC) is an important, generally non-invasive, bacterial pathogen that causes diarrhea in humans. The microbe infects mainly the enterocytes of the small intestine. Here we have applied our newly developed infrared surface plasmon resonance (IR-SPR) spectroscopy approach to study how EPEC infection affects epithelial host cells. The IR-SPR experiments showed that EPEC infection results in a robust reduction in the refractive index of the infected cells. Assisted by confocal and total internal reflection microscopy, we discovered that the microbe dilates the intercellular gaps and induces the appearance of fluid-phase-filled pinocytic vesicles in the lower basolateral regions of the host epithelial cells. Partial cell detachment from the underlying substratum was also observed. Finally, the waveguide mode observed by our IR-SPR analyses showed that EPEC infection decreases the host cell's height to some extent. Together, these observations reveal novel impacts of the pathogen on the host cell architecture and endocytic functions. We suggest that these changes may induce the infiltration of a watery environment into the host cell, and potentially lead to failure of the epithelium barrier functions. Our findings also indicate the great potential of the label-free IR-SPR approach to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

שפה מקוריתאנגלית אמריקאית
מספר המאמרe78431
כתב עתPLoS ONE
כרך8
מספר גיליון10
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 23 אוק׳ 2013

ASJC Scopus subject areas

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להלן מוצגים תחומי המחקר של הפרסום 'Real-Time Sensing of Enteropathogenic E. coli-Induced Effects on Epithelial Host Cell Height, Cell-Substrate Interactions, and Endocytic Processes by Infrared Surface Plasmon Spectroscopy'. יחד הם יוצרים טביעת אצבע ייחודית.

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