Matrin 3 binds and stabilizes mRNA

Maayan Salton, Ran Elkon, Tatiana Borodina, Aleksey Davydov, Marie Laure Yaspo, Eran Halperin, Yosef Shiloh

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

Matrin 3 (MATR3) is a highly conserved, inner nuclear matrix protein with two zinc finger domains and two RNA recognition motifs (RRM), whose function is largely unknown. Recently we found MATR3 to be phosphorylated by the protein kinase ATM, which activates the cellular response to double strand breaks in the DNA. Here, we show that MATR3 interacts in an RNA-dependent manner with several proteins with established roles in RNA processing, and maintains its interaction with RNA via its RRM2 domain. Deep sequencing of the bound RNA (RIP-seq) identified several small noncoding RNA species. Using microarray analysis to explore MATR3′s role in transcription, we identified 77 transcripts whose amounts depended on the presence of MATR3. We validated this finding with nine transcripts which were also bound to the MATR3 complex. Finally, we demonstrated the importance of MATR3 for maintaining the stability of several of these mRNA species and conclude that it has a role in mRNA stabilization. The data suggest that the cellular level of MATR3, known to be highly regulated, modulates the stability of a group of gene transcripts.

שפה מקוריתאנגלית אמריקאית
מספר המאמרe23882
כתב עתPLoS ONE
כרך6
מספר גיליון8
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 2011

ASJC Scopus subject areas

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