Longitudinal Relaxation Enhancement in H-1 NMR Spectroscopy of Tissue Metabolites via Spectrally Selective Excitation

Noam Shemesh, Jean-Nicolas Dumez, Lucio Frydman

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

Nuclear magnetic resonance spectroscopy is governed by longitudinal (T-1) relaxation. For protein and nucleic acid experiments in solutions, it is well established that apparent T-1 values can be enhanced by selective excitation of targeted resonances. The present study explores such longitudinal relaxation enhancement (LRE) effects for molecules residing in biological tissues. The longitudinal relaxation recovery of tissue resonances positioned both down- and upfield of the water peak were measured by spectrally selective excitation/refocusing pulses, and compared with conventional water-suppressed, broadband-excited counterparts at 9.4T. Marked LRE effects with up to threefold reductions in apparent T-1 values were observed as expected for resonances in the 6-9ppm region; remarkably, statistically significant LRE effects were also found for several non-exchanging metabolite resonances in the 1-4ppm region, encompassing 30-50% decreases in apparent T-1 values. These LRE effects suggest a novel means of increasing the sensitivity of tissue-oriented experiments, and open new vistas to investigate the nature of interactions among metabolites, water and macromolecules at a molecular level.
שפה מקוריתאנגלית
עמודים (מ-עד)13002-13008
מספר עמודים7
כתב עתChemistry-A European Journal
כרך19
מספר גיליון39
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - ספט׳ 2013

טביעת אצבע

להלן מוצגים תחומי המחקר של הפרסום 'Longitudinal Relaxation Enhancement in H-1 NMR Spectroscopy of Tissue Metabolites via Spectrally Selective Excitation'. יחד הם יוצרים טביעת אצבע ייחודית.

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