Deciphering the Arginine-Binding preferences at the Substrate-Binding groove of Ser/Thr kinases by computational surface mapping

Avraham Ben-Shimon, Masha Y. Niv

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

Protein kinases are key signaling enzymes that catalyze the transfer of γ-phosphate from an ATP molecule to a phospho-accepting residue in the substrate. Unraveling the molecular features that govern the preference of kinases for particular residues flanking the phosphoacceptor is important for understanding kinase specificities toward their substrates and for designing substrate-like peptidic inhibitors. We applied ANCHORSmap, a new fragment-based computational approach for mapping amino acid side chains on protein surfaces, to predict and characterize the preference of kinases toward Arginine binding. We focus on positions P-2 and P-5, commonly occupied by Arginine (Arg) in substrates of basophilic Ser/Thr kinases. The method accurately identified all the P-2/P-5 Arg binding sites previously determined by X-ray crystallography and produced Arg preferences that corresponded to those experimentally found by peptide arrays. The predicted Arg-binding positions and their associated pockets were analyzed in terms of shape, physicochemical properties, amino acid composition, and in-silico mutagenesis, providing structural rationalization for previously unexplained trends in kinase preferences toward Arg moieties. This methodology sheds light on several kinases that were described in the literature as having non-trivial preferences for Arg, and provides some surprising departures from the prevailing views regarding residues that determine kinase specificity toward Arg. In particular, we found that the preference for a P-5 Arg is not necessarily governed by the 170/230 acidic pair, as was previously assumed, but by several different pairs of acidic residues, selected from positions 133, 169, and 230 (PKA numbering). The acidic residue at position 230 serves as a pivotal element in recognizing Arg from both the P-2 and P-5 positions.

שפה מקוריתאנגלית אמריקאית
מספר המאמרe1002288
כתב עתPLoS Computational Biology
כרך7
מספר גיליון11
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - נוב׳ 2011

ASJC Scopus subject areas

  • ???subjectarea.asjc.1100.1105???
  • ???subjectarea.asjc.2600.2611???
  • ???subjectarea.asjc.2300.2303???
  • ???subjectarea.asjc.1300.1312???
  • ???subjectarea.asjc.1300.1311???
  • ???subjectarea.asjc.2800.2804???
  • ???subjectarea.asjc.1700.1703???

טביעת אצבע

להלן מוצגים תחומי המחקר של הפרסום 'Deciphering the Arginine-Binding preferences at the Substrate-Binding groove of Ser/Thr kinases by computational surface mapping'. יחד הם יוצרים טביעת אצבע ייחודית.

פורמט ציטוט ביבליוגרפי