cSPARCOM: Multi-detector reconstruction by confocal super-resolution correlation microscopy: Multi-detector reconstruction by confocal super-resolution correlation microscopy

Uri Rossman, Tali Dadosh, Yonina Eldar, Dan Oron

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

Image scanning microscopy (ISM), an upgraded successor of the ubiquitous confocal microscope, facilitates up to two-fold improvement in lateral resolution, and has become an indispensable element in the toolbox of the bio-imaging community. Recently, super-resolution optical fluctuation image scanning microscopy (SOFISM) integrated the analysis of intensity-fluctuations information into the basic ISM architecture, to enhance its resolving power. Both of these techniques typically rely on pixel-reassignment as a fundamental processing step, in which the parallax of different detector elements to the sample is compensated by laterally shifting the point spread function (PSF). Here, we propose an alternative analysis approach, based on the recent high-performing sparsity-based super-resolution correlation microscopy (SPARCOM) method. Through measurements of DNA origami nano-rulers and fixed cells labeled with organic dye, we experimentally show that confocal SPARCOM (cSPARCOM), which circumvents pixel-reassignment altogether, provides enhanced resolution compared to pixel-reassigned based analysis. Thus, cSPARCOM further promotes the effectiveness of ISM, and particularly that of correlation based ISM implementations such as SOFISM, where the PSF deviates significantly from spatial invariance.
שפה מקוריתאנגלית
עמודים (מ-עד)12772-12786
מספר עמודים15
כתב עתOptics Express
כרך29
מספר גיליון9
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 26 אפר׳ 2021

ASJC Scopus subject areas

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