Conserved pleiotropy of an ancient plant homeobox gene uncovered by cis-regulatory dissection

Anat Hendelman, Sophia Zebell, Daniel Rodriguez-Leal, Noah Dukler, Gina Robitaille, Xuelin Wu, Jamie Kostyun, Lior Tal, Peipei Wang, Madelaine E Bartlett, Yuval Eshed, Idan Efroni, Zachary B Lippman

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

Divergence of gene function is a hallmark of evolution, but assessing functional divergence over deep time is not trivial. The few alleles available for cross-species studies often fail to expose the entire functional spectrum of genes, potentially obscuring deeply conserved pleiotropic roles. Here, we explore the functional divergence of WUSCHEL HOMEOBOX9 (WOX9), suggested to have species-specific roles in embryo and inflorescence development. Using a cis-regulatory editing drive system, we generate a comprehensive allelic series in tomato, which revealed hidden pleiotropic roles for WOX9. Analysis of accessible chromatin and conserved cis-regulatory sequences identifies the regions responsible for this pleiotropic activity, the functions of which are conserved in groundcherry, a tomato relative. Mimicking these alleles in Arabidopsis, distantly related to tomato and groundcherry, reveals new inflorescence phenotypes, exposing a deeply conserved pleiotropy. We suggest that targeted cis-regulatory mutations can uncover conserved gene functions and reduce undesirable effects in crop improvement.
שפה מקוריתאנגלית
עמודים (מ-עד)1724-1739
מספר עמודים17
כתב עתCell
כרך184
מספר גיליון7
תאריך מקוון מוקדם4 מרץ 2021
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 1 אפר׳ 2021

טביעת אצבע

להלן מוצגים תחומי המחקר של הפרסום 'Conserved pleiotropy of an ancient plant homeobox gene uncovered by cis-regulatory dissection'. יחד הם יוצרים טביעת אצבע ייחודית.

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