Biological Effect Tetra-Branched Anti-TNF-Peptide and Coating RatioDependent Penetration of the Peptide-Conjugated Cerium3/4+ Cation-StabilizedGamma-Maghemite Nanoparticles into Rat Inner Ear after TranstympanicInjection Visualized by MRI

J Zou, B Peng, S Ostrovsky, B Li, C Li, MI Kettunen, JP Lellouche, I Pyykkö

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

תקציר

Objective: To evaluate the biological efficacy of TBATP and their distribution in the inner ear after transtympanic injection. Methods: TBATP was synthesized through standard Fmoc solid phase synthesis. A model of TNF-α-induced apoptosis was established in human umbilical vein endothelial cells (HUVECs). The peptides were covalently attached onto CAN-γ-Fe2 O3 NPs. Nanoparticle suspension was injected to rat middle ear cavity. Distribution of CAN-γ-Fe2 O3 NPs in the inner ear was detected using a 7.0 T MRI machine in combination with Prussian blue staining. Results: TBATP almost fully suppressed the inhibitory effect on HUVECs induced by TNF-α while the linear one reduced less than half of the effect. CAN-γ-Fe2 O3 NPs conjugated to TBATP at a peptide weight ratio of 10% but not 50% efficiently entered the inner ear at 3 h through 2 w post-middle ear administrations and most pronounced at 2 w. Conclusion: The tetra-branched anti-TNF-α peptide (TBATP) is capable of suppressing the inhibitory effect on HUVECs induced by TNF-α, and is visualized by MRI after conjugating to super-paramagnetic Ce3/4+ cation-doped maghemite nanoparticles arising from ceric ammonium nitrate-mediated doping oxidation of starting magnetite nanoparticles (CAN-γ-Fe2 O3 NPs). Keywords: Peptide; Functional maghemite nanoparticles; Inner ear; Magnetic resonance imaging; Biological barrier. List of Abbreviations: CAN-γ-Fe2 O3 NPs: Super-paramagnetic maghemite (γ-Fe2 O3 ) nanoparticles using ceric ammonium nitrate (CAN)-mediated oxidation of starting magnetite (Fe3 O4 ) nanoparticles; HUVECs: Human umbilical vein endothelial cells; TNF-α: tumor necrosis factor-α.
שפה מקוריתאנגלית אמריקאית
מספר עמודים9
כתב עתJournal of Materials Science and Nanotechnology,
כרך5
מספר גיליון22
סטטוס פרסוםפורסם - 2017

טביעת אצבע

להלן מוצגים תחומי המחקר של הפרסום 'Biological Effect Tetra-Branched Anti-TNF-Peptide and Coating RatioDependent Penetration of the Peptide-Conjugated Cerium3/4+ Cation-StabilizedGamma-Maghemite Nanoparticles into Rat Inner Ear after TranstympanicInjection Visualized by MRI'. יחד הם יוצרים טביעת אצבע ייחודית.

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