Yeast phospholipid biosynthesis is linked to mRNA localization

Orit Hermesh, Christian Genz, Ido Yofe, Monika Sinzel, Doron Rapaport, Maya Schuldiner, Ralf-Peter Jansen

Research output: Contribution to journalArticlepeer-review

Abstract

Regulation of the localization of mRNAs and local translation are universal features in eukaryotes and contribute to cellular asymmetry and differentiation. In Saccharomyces cerevisiae, localization of mRNAs that encode membrane proteins requires the She protein machinery, including the RNA-binding protein She2p, as well as movement of the cortical endoplasmic reticulum (cER) to the yeast bud. In a screen for ER-specific proteins necessary for the directional transport of WSC2 and EAR1 mRNAs, we have identified enzymes that are involved in phospholipid metabolism. Loss of the phospholipid methyltransferase Cho2p, which showed the strongest impact on mRNA localization, disturbs mRNA localization, as well as ER morphology and segregation, owing to an increase in the amount of cellular phosphatidylethanolamine (PtdEtn). Mislocalized mRNPs containing She2p colocalize with aggregated cER structures, suggestive of the entrapment of mRNA and She2p by the elevated PtdEtn level. This was confirmed by the elevated binding of She2p to PtdEtn-containing liposomes. These findings underscore the importance of ER membrane integrity in mRNA transport.

Original languageEnglish
Pages (from-to)3373-3381
Number of pages9
JournalJournal of Cell Science
Volume127
Issue number15
DOIs
StatePublished - 2014

All Science Journal Classification (ASJC) codes

  • Cell Biology

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