TY - JOUR
T1 - WRKY1 acts as a key component improving resistance against Alternaria solani in wild tomato, Solanum arcanum Peralta
AU - Shinde, Balkrishna A.
AU - Dholakia, Bhushan B.
AU - Hussain, Khalid
AU - Aharoni, Asaph
AU - Giri, Ashok P.
AU - Kamble, Avinash C.
N1 - BAS is thankful to University Grant Commission (UGC) New Delhi, India, for SRF fellowship. ACK and APG acknowledge financial support from Council of Scientific and Industrial Research (CSIR), New Delhi, India, under project code, 60(0101)/11/EMR-II and XII 5-year plan project BSC0107, respectively. Authors thank Savitribai Phule Pune University for financial support under UPEII program [UGC 262 (B)(3)], Dr. Oren Tzfadia (Weizmann Institute of Science, Israel) for co-expression analysis, Dr. Vitthal Barvkar for helping in promoter analysis and Dr. Suresh Bhat (CSIR-NCL) for confocal microscope facility.
PY - 2018/8
Y1 - 2018/8
N2 - Early blight (EB), caused by Alternaria solani, is a major threat to global tomato production. In comparison with cultivated tomato (Solanum lycopersicum), a wild relative, S. arcanum exhibits strong resistance against EB. However, molecular cascades operating during EB resistance in wild or cultivated tomato plants are largely obscure. Here, we provide novel insight into spatio-temporal molecular events in S. arcanum against A. solani. Transcriptome and co-expression analysis presented 33-WRKYs as promising candidates of which 12 SaWRKYs displayed differential expression patterns in resistant and susceptible accessions during EB disease progression. Among these, SaWRKY1 exhibited induced expression with significant modulation in xyloglucan endotrans hydrolase 5 (XTH5) and MYB2 expressions that correlated with the disease phenotypes. Electro-mobility shift assay confirmed physical interaction of recombinant SaWRKY1 to SaXTH5 and SaMYB2 promoters. Comparative WRKY1 promoter analysis between resistant and susceptible plants revealed the presence of crucial motifs for defence mechanism exclusively in resistant accession. Additionally, many defence-related genes displayed significant expression variations in both the accessions. Further, WRKY1 overexpressing transgenic plants exhibited higher levels of EB resistance while RNAi silencing lines had increased susceptibility to A. solani with altered expression of XTH5 and MYB2. Overall, these findings demonstrate the positive influence of WRKY1 in improving EB resistance in wild tomato and this could be further utilized as a potential target through genetic engineering to augment protection against A. solani in crop plants.
AB - Early blight (EB), caused by Alternaria solani, is a major threat to global tomato production. In comparison with cultivated tomato (Solanum lycopersicum), a wild relative, S. arcanum exhibits strong resistance against EB. However, molecular cascades operating during EB resistance in wild or cultivated tomato plants are largely obscure. Here, we provide novel insight into spatio-temporal molecular events in S. arcanum against A. solani. Transcriptome and co-expression analysis presented 33-WRKYs as promising candidates of which 12 SaWRKYs displayed differential expression patterns in resistant and susceptible accessions during EB disease progression. Among these, SaWRKY1 exhibited induced expression with significant modulation in xyloglucan endotrans hydrolase 5 (XTH5) and MYB2 expressions that correlated with the disease phenotypes. Electro-mobility shift assay confirmed physical interaction of recombinant SaWRKY1 to SaXTH5 and SaMYB2 promoters. Comparative WRKY1 promoter analysis between resistant and susceptible plants revealed the presence of crucial motifs for defence mechanism exclusively in resistant accession. Additionally, many defence-related genes displayed significant expression variations in both the accessions. Further, WRKY1 overexpressing transgenic plants exhibited higher levels of EB resistance while RNAi silencing lines had increased susceptibility to A. solani with altered expression of XTH5 and MYB2. Overall, these findings demonstrate the positive influence of WRKY1 in improving EB resistance in wild tomato and this could be further utilized as a potential target through genetic engineering to augment protection against A. solani in crop plants.
U2 - https://doi.org/10.1111/pbi.12892
DO - https://doi.org/10.1111/pbi.12892
M3 - مقالة
C2 - 29377467
SN - 1467-7644
VL - 16
SP - 1502
EP - 1513
JO - Plant Biotechnology Journal
JF - Plant Biotechnology Journal
IS - 8
ER -