TY - JOUR
T1 - Visualizing the structure and motion of the long noncoding RNA HOTAIR
AU - Spokoini-Stern, Rachel
AU - Stamov, Dimitar
AU - Jessel, Hadass
AU - Aharoni, Lior
AU - Haschke, Heiko
AU - Giron, Jonathan
AU - Unger, Ron
AU - Segal, Eran
AU - Abu-Horowitz, Almogit
AU - Bachelet, Ido
N1 - Publisher Copyright: © 2020 Spokoini-Stern et al. This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
PY - 2020/5
Y1 - 2020/5
N2 - Long noncoding RNA molecules (lncRNAs) are estimated to account for the majority of eukaryotic genomic transcripts, and have been associated with multiple diseases in humans. However, our understanding of their structure-function relationships is scarce, with structural evidence coming mostly from indirect biochemical approaches or computational predictions. Here we describe direct visualization of the lncRNA HOTAIR (HOx Transcript AntIsense RNA) using atomic force microscopy (AFM) in nucleus-like conditions at 37°. Our observations reveal that HOTAIR has a discernible, although flexible, shape. Fast AFM scanning enabled the quantification of the motion of HOTAIR, and provided visual evidence of physical interactions with genomic DNA segments. Our report provides a biologically plausible description of the anatomy and intrinsic properties of HOTAIR, and presents a framework for studying the structural biology of lncRNAs.
AB - Long noncoding RNA molecules (lncRNAs) are estimated to account for the majority of eukaryotic genomic transcripts, and have been associated with multiple diseases in humans. However, our understanding of their structure-function relationships is scarce, with structural evidence coming mostly from indirect biochemical approaches or computational predictions. Here we describe direct visualization of the lncRNA HOTAIR (HOx Transcript AntIsense RNA) using atomic force microscopy (AFM) in nucleus-like conditions at 37°. Our observations reveal that HOTAIR has a discernible, although flexible, shape. Fast AFM scanning enabled the quantification of the motion of HOTAIR, and provided visual evidence of physical interactions with genomic DNA segments. Our report provides a biologically plausible description of the anatomy and intrinsic properties of HOTAIR, and presents a framework for studying the structural biology of lncRNAs.
KW - Atomic force microscopy
KW - HOTAIR
KW - Long noncoding RNA
UR - http://www.scopus.com/inward/record.url?scp=85083623218&partnerID=8YFLogxK
U2 - 10.1261/rna.074633.120
DO - 10.1261/rna.074633.120
M3 - مقالة
C2 - 32115425
SN - 1355-8382
VL - 26
SP - 629
EP - 636
JO - RNA
JF - RNA
IS - 5
ER -