Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates

Francois Fernand; Liel Rubinsky; Alex Golberg; Boris Rubinsky

doi:10.1002/bit.24479

Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates

Francois Fernand, Liel Rubinsky, Alex Golberg, Boris Rubinsky

Research output: Contribution to journal › Article › peer-review

Abstract

The mathematical solution to the electric field equation in cylindrical coordinates, has suggested to us a new experimental methodology and device for reducing experimental effort in designing electroporation protocols. Using a new cylindrical electroporation system, we show, with an Escherichia coli cell model, how key electroporation parameters emerge precisely from single experiments rather than through interpolation from numerous experiments in the conventional Cartesian electroporation system.

Original language	English
Pages (from-to)	2168-2171
Number of pages	4
Journal	Biotechnology and Bioengineering
Volume	109
Issue number	8
DOIs	https://doi.org/10.1002/bit.24479
State	Published - Aug 2012
Externally published	Yes

Keywords

Electroporation
High throughput
Protocol design

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/bit.24479

Cite this

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title = "Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates",

abstract = "The mathematical solution to the electric field equation in cylindrical coordinates, has suggested to us a new experimental methodology and device for reducing experimental effort in designing electroporation protocols. Using a new cylindrical electroporation system, we show, with an Escherichia coli cell model, how key electroporation parameters emerge precisely from single experiments rather than through interpolation from numerous experiments in the conventional Cartesian electroporation system.",

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author = "Francois Fernand and Liel Rubinsky and Alex Golberg and Boris Rubinsky",

year = "2012",

month = aug,

doi = "10.1002/bit.24479",

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AU - Fernand, Francois

AU - Rubinsky, Liel

AU - Golberg, Alex

AU - Rubinsky, Boris

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AB - The mathematical solution to the electric field equation in cylindrical coordinates, has suggested to us a new experimental methodology and device for reducing experimental effort in designing electroporation protocols. Using a new cylindrical electroporation system, we show, with an Escherichia coli cell model, how key electroporation parameters emerge precisely from single experiments rather than through interpolation from numerous experiments in the conventional Cartesian electroporation system.

KW - Electroporation

KW - High throughput

KW - Protocol design

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DO - 10.1002/bit.24479

M3 - مقالة

SN - 0006-3592

VL - 109

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EP - 2171

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

IS - 8

ER -

Variable electric fields for high throughput electroporation protocol design in curvilinear coordinates

Abstract

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

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