Validation of two commercial real-time PCR assays for rapid screening of the HLA-B*57:01 allele in the HIV clinical laboratory

Boaz Avidor, Shirley Girshengorn, Liran Giladi, Shoshana Israel, Rina Katz, Dan Turner

Research output: Contribution to journalArticlepeer-review

Abstract

The pharmacogenetics approach to screen for the presence of the HLA-B*57:01 allele in HIV-1 infected patients is mandatory to prevent the potential development of hypersensitivity reaction to abacavir treatment. Given the limitations of current genotype methodologies, commercial real-time PCR assays were specifically developed for this purpose, but have not been sufficiently validated and are still not widely used. Here, in the context of the HIV laboratory, we assessed the ability of two commercial kits, the LightSNiP rs2395029 HPC5 assay (TIB Molbiol) and the DuplicαReal-Time HLA-B*5701 Genotyping kit (Euroclone), to retrospectively detect HLA-B*57:01 positive and negative samples of Israeli HIV-1 infected patients. The LightSNiP rs2395029 HPC5 assay had false-positive results, whereas the DuplicαReal-Time HLA-B*5701 Genotyping kit was highly accurate and could be readily implemented into clinical practice. It is hoped that this study will facilitate the assessment of additional commercial kits for HLA-B*57:01 detection and expand their use in the clinical laboratory. Such studies can likely help the use of abacavir treatment in HIV-1 infected patients.

Original languageEnglish
Pages (from-to)18-24
Number of pages7
JournalJournal of Virological Methods
Volume237
DOIs
StatePublished - 1 Nov 2016

Keywords

  • Abacavir hypersensitivity
  • HIV laboratory
  • HIV-1
  • HLA-B*57:01 screening
  • Real-time PCR

All Science Journal Classification (ASJC) codes

  • Virology

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