Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: Implications for the localization of mRNAs by MS2-MCP system"

Jeffrey Gerst; Gal Haimovich; D Zabezhinski; B. Haas; Boris Slobodin; Pravinkumar Purushothaman; L. Fan; J.Z. Levin; C Nusbaum; Dmitry Zabezhinsky

doi:https://doi.org/10.1261/rna.055095.115

Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: Implications for the localization of mRNAs by MS2-MCP system"

Jeffrey Gerst, Gal Haimovich, D Zabezhinski, B. Haas, Boris Slobodin, Pravinkumar Purushothaman, L. Fan, J.Z. Levin, C Nusbaum, Dmitry Zabezhinsky

Department of Molecular Genetics

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

The MS2 system has been extensively used to visualize single mRNA molecules in live cells and follow their localization and behavior. In their Letter to the Editor recently published, Garcia and Parker suggest that use of the MS2 system may yield erroneous mRNA localization results due to the accumulation of 3′ decay products. Here we cite published works and provide new data which demonstrate that this is not a phenomenon general to endogenously expressed MS2-tagged transcripts, and that some of the results obtained in their study could have arisen from artifacts of gene expression.

Original language	English
Pages (from-to)	660-666
Number of pages	7
Journal	RNA
Volume	22
Issue number	5
DOIs	https://doi.org/10.1261/rna.055095.115
State	Published - May 2016

Keywords

MS2 aptamer
MS2 coat protein
P-bodies
mRNA decay
mRNA localization

All Science Journal Classification (ASJC) codes

Molecular Biology

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Gerst, J., Haimovich, G., Zabezhinski, D., Haas, B., Slobodin, B., Purushothaman, P., Fan, L., Levin, J. Z., Nusbaum, C., & Zabezhinsky, D. (2016). Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: Implications for the localization of mRNAs by MS2-MCP system". RNA, 22(5), 660-666. https://doi.org/10.1261/rna.055095.115

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abstract = "The MS2 system has been extensively used to visualize single mRNA molecules in live cells and follow their localization and behavior. In their Letter to the Editor recently published, Garcia and Parker suggest that use of the MS2 system may yield erroneous mRNA localization results due to the accumulation of 3′ decay products. Here we cite published works and provide new data which demonstrate that this is not a phenomenon general to endogenously expressed MS2-tagged transcripts, and that some of the results obtained in their study could have arisen from artifacts of gene expression.",

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Gerst, J, Haimovich, G, Zabezhinski, D, Haas, B, Slobodin, B, Purushothaman, P, Fan, L, Levin, JZ, Nusbaum, C & Zabezhinsky, D 2016, 'Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: Implications for the localization of mRNAs by MS2-MCP system"', RNA, vol. 22, no. 5, pp. 660-666. https://doi.org/10.1261/rna.055095.115

Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: Implications for the localization of mRNAs by MS2-MCP system". / Gerst, Jeffrey; Haimovich, Gal; Zabezhinski, D et al.
In: RNA, Vol. 22, No. 5, 05.2016, p. 660-666.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Use of the MS2 aptamer and coat protein for RNA localization in yeast

T2 - A response to "MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: Implications for the localization of mRNAs by MS2-MCP system"

AU - Gerst, Jeffrey

AU - Haimovich, Gal

AU - Zabezhinski, D

AU - Haas, B.

AU - Slobodin, Boris

AU - Purushothaman, Pravinkumar

AU - Fan, L.

AU - Levin, J.Z.

AU - Nusbaum, C

AU - Zabezhinsky, Dmitry

PY - 2016/5

Y1 - 2016/5

N2 - The MS2 system has been extensively used to visualize single mRNA molecules in live cells and follow their localization and behavior. In their Letter to the Editor recently published, Garcia and Parker suggest that use of the MS2 system may yield erroneous mRNA localization results due to the accumulation of 3′ decay products. Here we cite published works and provide new data which demonstrate that this is not a phenomenon general to endogenously expressed MS2-tagged transcripts, and that some of the results obtained in their study could have arisen from artifacts of gene expression.

AB - The MS2 system has been extensively used to visualize single mRNA molecules in live cells and follow their localization and behavior. In their Letter to the Editor recently published, Garcia and Parker suggest that use of the MS2 system may yield erroneous mRNA localization results due to the accumulation of 3′ decay products. Here we cite published works and provide new data which demonstrate that this is not a phenomenon general to endogenously expressed MS2-tagged transcripts, and that some of the results obtained in their study could have arisen from artifacts of gene expression.

KW - MS2 aptamer

KW - MS2 coat protein

KW - P-bodies

KW - mRNA decay

KW - mRNA localization

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DO - https://doi.org/10.1261/rna.055095.115

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SN - 1355-8382

VL - 22

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EP - 666

JO - RNA

JF - RNA

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ER -

Gerst J, Haimovich G, Zabezhinski D, Haas B, Slobodin B, Purushothaman P et al. Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: Implications for the localization of mRNAs by MS2-MCP system". RNA. 2016 May;22(5):660-666. doi: https://doi.org/10.1261/rna.055095.115

Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to "MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: Implications for the localization of mRNAs by MS2-MCP system"

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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