@inbook{2b356594749b478992a63ec81ce60106,
title = "UPLC-MS-based metabolite analysis in tomato",
abstract = "Recent advances in the performance of hyphenated technologies based on ultrapressure chromatography and high-sensitivity mass spectrometry have set the stage for a myriad of metabolomics studies in plants and other organisms. In this chapter, we describe the use of a UPLC (Ultraperformance Liquid Chromatography)-qTOF (quadrupole time-of-flight) system for profiling semipolar metabolites in the model fruit plant tomato. An optimized extraction method, instrument parameters and data treatment procedures are provided. The value of UPLC instruments, which use small particle size chromatographic columns, in terms of resolution, separation, and short injection times are presented. When coupled to a TOF mass spectrometer with high resolution and mass accuracy, good dynamic range, and a fast spectral acquisition capacity, this system is most suitable for the extensive profiling of hundreds of plant metabolites.",
author = "Ilana Rogachev and Asaph Aharoni",
note = "We are grateful to Arye Tishbee for operating the UPLC-qTOF instrument, Max Itkin and Sagit Meir for the assistance in the preparation of tomato extracts and Ilya Venger with XCMS analysis. A.A. is an incumbent of the Adolfo and Evelyn Blum Career Development Chair. The research in the A.A. laboratory was supported by the William Z. and Eda Bess Novick Young Scientist Fund, the Y. Leon Benoziyo Institute for Molecular Medicine and the EU project “META-PHOR” contract number FOODCT-2006-036220.",
year = "2012",
month = mar,
day = "16",
doi = "10.1007/978-1-61779-594-7_9",
language = "الإنجليزيّة",
isbn = "9781617795930",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "129--144",
editor = "Nigel Hardy and Robert Hall",
booktitle = "Plant Metabolomics",
address = "الولايات المتّحدة",
}