Abstract
Advances in native mass spectrometry and single-molecule techniques have made it possible in recent years to determine the values of successive ligand binding constants for large multi-subunit proteins. Given these values, it is possible to distinguish between different allosteric mechanisms and, thus, obtain insights into how various bio-molecular machines work. Here, we describe for ring-shaped homo-oligomers, in particular, how the relationship between the values of successive ligand binding constants is diagnostic for concerted, sequential and probabilistic allosteric mechanisms.
This article is part of a discussion meeting issue 'Allostery and molecular machines'.
Original language | English |
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Article number | 20170176 |
Number of pages | 5 |
Journal | Philosophical Transactions of the Royal Society B: Biological Sciences |
Volume | 373 |
Issue number | 1749 |
DOIs | |
State | Published - 19 Jun 2018 |