Unpicking allosteric mechanisms of homo-oligomeric proteins by determining their successive ligand binding constants

Ranit Gruber, Amnon Horovitz

Research output: Contribution to journalReview articlepeer-review

Abstract

Advances in native mass spectrometry and single-molecule techniques have made it possible in recent years to determine the values of successive ligand binding constants for large multi-subunit proteins. Given these values, it is possible to distinguish between different allosteric mechanisms and, thus, obtain insights into how various bio-molecular machines work. Here, we describe for ring-shaped homo-oligomers, in particular, how the relationship between the values of successive ligand binding constants is diagnostic for concerted, sequential and probabilistic allosteric mechanisms.

This article is part of a discussion meeting issue 'Allostery and molecular machines'.

Original languageEnglish
Article number20170176
Number of pages5
JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
Volume373
Issue number1749
DOIs
StatePublished - 19 Jun 2018

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