Unlocking trophectoderm mysteries: In vivo and in vitro perspectives on human and mouse trophoblast fate induction

Meir Azagury, Yosef Buganim

Research output: Contribution to journalReview articlepeer-review


In recent years, the pursuit of inducing the trophoblast stem cell (TSC) state has gained prominence as a compelling research objective, illuminating the establishment of the trophoblast lineage and unlocking insights into early embryogenesis. In this review, we examine how advancements in diverse technologies, including in vivo time course transcriptomics, cellular reprogramming to TSC state, chemical induction of totipotent stem-cell-like state, and stem-cell-based embryo-like structures, have enriched our insights into the intricate molecular mechanisms and signaling pathways that define the mouse and human trophectoderm/TSC states. We delve into disparities between mouse and human trophectoderm/TSC fate establishment, with a special emphasis on the intriguing role of pluripotency in this context. Additionally, we re-evaluate recent findings concerning the potential of totipotent-stem-like cells and embryo-like structures to fully manifest the trophectoderm/trophoblast lineage's capabilities. Lastly, we briefly discuss the potential applications of induced TSCs in pregnancy-related disease modeling.

Original languageAmerican English
Pages (from-to)941-960
Number of pages20
JournalDevelopmental Cell
Issue number8
StatePublished - 22 Apr 2024


  • ESC-TSC transdifferentiation
  • early embryogenesis
  • reprogramming
  • stem-cell-based embryos
  • totipotency induction
  • trophectoderm
  • trophoblast stem cells

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology
  • Molecular Biology
  • Cell Biology
  • Developmental Biology


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