UMI-4C for quantitative and targeted chromosomal contact profiling

Omer Schwartzman; Zohar Mukamel; Elkayam, Noa Oded Elkayam; Pedro Olivares-Chauvet; Yaniv Lubling; Gilad Landan; Shai Izraeli; M. -Z Lai; Amos Tanay

doi:10.1038/nmeth.3922

UMI-4C for quantitative and targeted chromosomal contact profiling

Omer Schwartzman, Zohar Mukamel, Elkayam, Noa Oded Elkayam, Pedro Olivares-Chauvet, Yaniv Lubling, Gilad Landan, Shai Izraeli, M. -Z Lai, Amos Tanay

Weizmann Institute of Science, Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (UMIs) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can easily be multiplexed to allow robust comparison of contact distributions between loci and conditions. This approach may open the way to the incorporation of contact distributions into quantitative models of gene regulation.

Original language	English
Pages (from-to)	685-691
Number of pages	7
Journal	Nature Methods
Volume	13
Issue number	8
DOIs	https://doi.org/10.1038/nmeth.3922
State	Published - 28 Jul 2016

All Science Journal Classification (ASJC) codes

Molecular Biology
Biochemistry
Biotechnology
Cell Biology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/nmeth.3922

Cite this

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abstract = "We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (UMIs) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can easily be multiplexed to allow robust comparison of contact distributions between loci and conditions. This approach may open the way to the incorporation of contact distributions into quantitative models of gene regulation.",

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AU - Schwartzman, Omer

AU - Mukamel, Zohar

AU - Oded Elkayam, Elkayam, Noa

AU - Olivares-Chauvet, Pedro

AU - Lubling, Yaniv

AU - Landan, Gilad

AU - Izraeli, Shai

AU - Lai, M. -Z

AU - Tanay, Amos

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N2 - We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (UMIs) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can easily be multiplexed to allow robust comparison of contact distributions between loci and conditions. This approach may open the way to the incorporation of contact distributions into quantitative models of gene regulation.

AB - We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (UMIs) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can easily be multiplexed to allow robust comparison of contact distributions between loci and conditions. This approach may open the way to the incorporation of contact distributions into quantitative models of gene regulation.

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M3 - مقالة

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JO - Nature Methods

JF - Nature Methods

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ER -

UMI-4C for quantitative and targeted chromosomal contact profiling

Abstract

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