Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD

Ryan A. Colyer, Giuseppe Scalia, Federica A. Villa, Fabrizio Guerrieri, Simone Tisa, Franco Zappa, Sergio Cova, Shimon Weiss, Xavier Michalet

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Single-molecule spectroscopy is a powerful approach to measuring molecular properties such as size, brightness, conformation, and binding constants. Due to the low concentrations in the single-molecule regime, measurements with good statistical accuracy require long acquisition times. Previously we showed a factor of 8 improvement in acquisition speed using a custom-CMOS 8x1 SPAD array. Here we present preliminary results with a 64X improvement in throughput obtained using a liquid crystal on silicon spatial light modulator (LCOS-SLM) and a novel standard CMOS 1024 pixel SPAD array, opening the way to truly high-throughput single-molecule spectroscopy.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Imaging IV
DOIs
StatePublished - 28 Feb 2011
Externally publishedYes
EventSingle Molecule Spectroscopy and Imaging IV - San Francisco, CA, United States
Duration: 22 Jan 201123 Jan 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7905

Conference

ConferenceSingle Molecule Spectroscopy and Imaging IV
Country/TerritoryUnited States
CitySan Francisco, CA
Period22/01/1123/01/11

Keywords

  • CMOS
  • FCS
  • LCOS
  • SPAD array
  • fluorescence
  • high-throughput
  • photon counting
  • single-molecule

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint

Dive into the research topics of 'Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD'. Together they form a unique fingerprint.

Cite this