Translatome Profiling of Tissue-Resident Macrophages Using the RiboTag Approach

Jung Seok Kim, Zhana Haimon, Sigalit Boura-Halfon, Steffen Jung

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Global gene expression profiling has provided valuable insights into the specific contributions of different cell types to various physiological processes. Notably though, both bulk and single-cell transcriptomics require the prior retrieval of the cells from their tissue context to be analyzed. Isolation protocols for tissue macrophages are, however, notoriously inefficient and, moreover, prone to introduce considerable bias and artifacts. Here, we will discuss a valuable alternative, originally introduced by Amieux and colleagues. This so-called RiboTag approach allows, in combination with respective macrophage-specific Cre transgenic lines, to retrieve macrophage translatomes from crude tissue extracts. We will review our experience with this ingenious method, focusing on the study of brain macrophages, including microglia and border-associated cells. We will elaborate on the advantages of the RiboTag approach that render it a valuable complement to standard cell sorting-based profiling strategies, especially for the investigation of tissue macrophages.

Original languageEnglish
Title of host publicationTissue-Resident Macrophages
Subtitle of host publicationMethods and Protocols
EditorsElvira Mass
PublisherHumana Press
Number of pages15
ISBN (Electronic)978-1-0716-3437-0
ISBN (Print)978-1-0716-3439-4
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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