Translation efficiency of synaptic proteins and its coding sequence determinants

Shelly Mahlab, Itai Linial, Michal Linial

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The synapse is an organized structure that contains synaptic vesicles, mitochondria, receptors, transporters and stored proteins. About 10% of the mRNAs that are express in mammalian neurons are delivered to synaptic sites, where they are subjected to local translation. While neuronal plasticity, learning and memory occur at the synapse, the mechanisms that regulate post-transcriptional events and local translation are mostly unknown. We hypothesized that evolutional signals that govern translational efficiency are encoded in the mRNA of synaptic proteins. Specifically, we applied a measure of tRNA adaptation index (tAI) as an indirect proxy for translation rate and showed that ionic channels and ligand-binding receptors are specified by a global low tAI values. In contrast, the genuine proteins of the synaptic vesicles exhibit significantly higher tAI values. The expression of many of these proteins actually accompanied synaptic plasticity. Furthermore, in human, the local tAI values for the initial segment of mRNA coding differs for synaptic proteins in view of the rest of the human proteome. We propose that the translation of synaptic proteins is a robust solution for compiling with the high metabolic demands of the synapse.

Original languageAmerican English
Title of host publicationBIOINFORMATICS 2013 - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms
Pages151-157
Number of pages7
StatePublished - 2013
EventInternational Conference on Bioinformatics Models, Methods and Algorithms, BIOINFORMATICS 2013 - Barcelona, Spain
Duration: 11 Feb 201314 Feb 2013

Publication series

NameBIOINFORMATICS 2013 - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms

Conference

ConferenceInternational Conference on Bioinformatics Models, Methods and Algorithms, BIOINFORMATICS 2013
Country/TerritorySpain
CityBarcelona
Period11/02/1314/02/13

Keywords

  • Codon usage
  • Dendrites
  • Endocytosis
  • Local translation
  • Neuron
  • TRNA adaptation
  • Translation rate

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Health Informatics
  • Modelling and Simulation

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