@article{5d3e00e5829a481fa96d5b3ef82e71d8,
title = "Transfer of the Septin Ring to Cytokinetic Remnants in ER Stress Directs Age-Sensitive Cell-Cycle Re-entry",
abstract = "During cell division, the inheritance of a functional endoplasmic reticulum (ER) is ensured by the endoplasmic reticulum stress surveillance (ERSU) pathway. Activation of ERSU causes the septin ring to mislocalize, which blocks ER inheritance and cytokinesis. Here, we uncover that the septin ring in fact translocates to previously utilized cell division sites called cytokinetic remnants (CRMs). This unconventional translocation requires Nba1, a negative polarity regulator that normally prevents repolarization and re-budding at CRMs. Furthermore, septin ring translocation relies on the recruitment and activation of a key ERSU component Slt2 by Beml , without activating Cdc42. Failure to transfer all septin subunits to CRMs delays the cell's ability to re-enter the cell cycle when ER homeostasis is restored and hinders cell growth after ER stress recovery. Thus, these deliberate but unprecedented rearrangements of cell polarity factors during ER stress safeguard cell survival and the timely cell-cycle re-entry upon ER stress recovery.",
author = "Chao, {Jesse T.} and Francisco Pina and Masayuki Onishi and Yifat Cohen and Ya-Shiuan Lai and Maya Schuldiner and Maho Niwa",
note = "We thank Dr. Stephan Michnick for the DHFR libraries. The work was supported by grants from the NIH (R01GM087415), UCSD Chancellor Excellent Scholarship (formally FISP), and the UCSD Academic Senate fund to M.N.; and by grants from Volkswagen Foundation “Life” grant and a The Deutsche Forschungsgemeinschaft (DFG) grant (SFB1190) to M.S. J.T.C. and F.P. were supported by a UCSD Molecular Biology Cancer postdoctoral fellowship and an NIH 5T32AI007469-20UCSD/LIAI allergy postdoctoral training grant, respectively. M.S. is an incumbent of the Dr. Gilbert Omenn and Martha Darling Professorial Chair In Molecular Genetics. We also thank Jon Mulholland and John Perrino (Stanford University Cell Sciences Imaging Facility) for assistance with electron microscopy. Author Contributions M.N. conceived the idea, analyzed the results, guided and organized the work, and wrote the manuscript. J.T.C. conceived the idea, performed the experiments, analyzed the data, and wrote the manuscript. M.S. proposed, analyzed the DHFR experiment, and provided advice on data interpretation. Y.C. performed the DHFR experiment and the data analyses. M.O. performed the EM experiments and provided general advice on the yeast septin, septum, and cytokinesis. F.P. performed a significant portion of the experiments, and Y.-S.L. performed additional experiments during the review process. Funding secured by M.N. All authors commented.",
year = "2019",
month = oct,
day = "21",
doi = "https://doi.org/10.1016/j.devcel.2019.08.017",
language = "الإنجليزيّة",
volume = "51",
pages = "173--191",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "2",
}