Tracking Root Interactions System (TRIS) Experiment and Quality Control

Hassan Massalha; Elisa Korenblum; Orr H. Shapiro; Asaph Aharoni

doi:https://doi.org/10.21769/BioProtoc.3211

Tracking Root Interactions System (TRIS) Experiment and Quality Control

Hassan Massalha, Elisa Korenblum, Orr H. Shapiro, Asaph Aharoni

Research output: Contribution to journal › Article › peer-review

Abstract

Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high spatial resolution is currently limited due to methodological intricacy. In this study, we developed a novel microfluidics-based device enabling direct imaging of root-bacteria interactions in real time.

Original language	American English
Article number	e3211
Journal	BIO-PROTOCOL
Volume	9
Issue number	8
DOIs	https://doi.org/10.21769/BioProtoc.3211
State	Published - 1 Jan 2019
Externally published	Yes

Keywords

Arabidopsis thaliana
Bacillus subtilis
Live-imaging microscopy
Microbial community dynamics
Root-bacteria interactions
Tracking root interactions system (TRIS)

All Science Journal Classification (ASJC) codes

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology
Plant Science

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https://doi.org/10.21769/BioProtoc.3211

Cite this

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title = "Tracking Root Interactions System (TRIS) Experiment and Quality Control",

abstract = "Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high spatial resolution is currently limited due to methodological intricacy. In this study, we developed a novel microfluidics-based device enabling direct imaging of root-bacteria interactions in real time.",

keywords = "Arabidopsis thaliana, Bacillus subtilis, Live-imaging microscopy, Microbial community dynamics, Root-bacteria interactions, Tracking root interactions system (TRIS)",

author = "Hassan Massalha and Elisa Korenblum and Shapiro, {Orr H.} and Asaph Aharoni",

year = "2019",

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day = "1",

doi = "https://doi.org/10.21769/BioProtoc.3211",

language = "American English",

volume = "9",

journal = "BIO-PROTOCOL",

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T1 - Tracking Root Interactions System (TRIS) Experiment and Quality Control

AU - Massalha, Hassan

AU - Korenblum, Elisa

AU - Shapiro, Orr H.

AU - Aharoni, Asaph

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high spatial resolution is currently limited due to methodological intricacy. In this study, we developed a novel microfluidics-based device enabling direct imaging of root-bacteria interactions in real time.

AB - Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high spatial resolution is currently limited due to methodological intricacy. In this study, we developed a novel microfluidics-based device enabling direct imaging of root-bacteria interactions in real time.

KW - Arabidopsis thaliana

KW - Bacillus subtilis

KW - Live-imaging microscopy

KW - Microbial community dynamics

KW - Root-bacteria interactions

KW - Tracking root interactions system (TRIS)

UR - http://www.scopus.com/inward/record.url?scp=85121293264&partnerID=8YFLogxK

U2 - https://doi.org/10.21769/BioProtoc.3211

DO - https://doi.org/10.21769/BioProtoc.3211

M3 - Article

SN - 2331-8325

VL - 9

JO - BIO-PROTOCOL

JF - BIO-PROTOCOL

IS - 8

M1 - e3211

ER -

Tracking Root Interactions System (TRIS) Experiment and Quality Control

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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