Tracking bacteria clearance using gold nanoparticles for wound therapy applications

Tamar Sadan; Guy Topaz; Rachela Popovtzer; Moris Topaz; Dror Fixler

doi:10.1117/12.2548441

Tracking bacteria clearance using gold nanoparticles for wound therapy applications

Tamar Sadan, Guy Topaz, Rachela Popovtzer, Moris Topaz, Dror Fixler

Bar-Ilan University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Bacterial infection and biofilm buildup is a major challenge for wound healing. In the present work we developed a method to track bacteria in vivo in order to analyze the progress of the healing. Regulated irrigation combined with negative pressure (RI-NPT) is a nonpharmaceutical therapeutic strategy for reducing bacterial load in acute and chronically infected wounds. Here, we analyzed RI-NPT hydrokinetics and efficacy of bacterial load reduction in wounds. Escherichia coli were loaded with gold nanoparticles, in order to make them "visible" and trackable using computed tomography (CT). Bacteria were tracked using CT in a low-flow rat wound model over time. CT tracking revealed that while regulated negative pressure-assisted wound therapy reduced bacterial load to a limited extent (5%), RI-NPT significantly increased bacterial outflow and clearance (by 45%). This nanotechnology-based approach demonstrates the ability to track bacteria in vivo at real time and also the ability of the RI-NPT technology to effectively reduce bacterial load.

Original language	English
Title of host publication	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII
Editors	Dror Fixler, Ewa M. Goldys, Sebastian Wachsmann-Hogiu
Publisher	SPIE
ISBN (Electronic)	9781510632714
DOIs	https://doi.org/10.1117/12.2548441
State	Published - 2020
Event	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII 2020 - San Francisco, United States Duration: 2 Feb 2020 → 3 Feb 2020

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11254

Conference

Conference	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII 2020
Country/Territory	United States
City	San Francisco
Period	2/02/20 → 3/02/20

Keywords

Bacterial clearance
Biofilm
Gold nanoparticles
Regulated irrigation and negative pressure-assisted wound therapy (RI-NPT)

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2548441

Cite this

Sadan, T., Topaz, G., Popovtzer, R., Topaz, M., & Fixler, D. (2020). Tracking bacteria clearance using gold nanoparticles for wound therapy applications. In D. Fixler, E. M. Goldys, & S. Wachsmann-Hogiu (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII Article 112541H (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11254). SPIE. https://doi.org/10.1117/12.2548441

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abstract = "Bacterial infection and biofilm buildup is a major challenge for wound healing. In the present work we developed a method to track bacteria in vivo in order to analyze the progress of the healing. Regulated irrigation combined with negative pressure (RI-NPT) is a nonpharmaceutical therapeutic strategy for reducing bacterial load in acute and chronically infected wounds. Here, we analyzed RI-NPT hydrokinetics and efficacy of bacterial load reduction in wounds. Escherichia coli were loaded with gold nanoparticles, in order to make them {"}visible{"} and trackable using computed tomography (CT). Bacteria were tracked using CT in a low-flow rat wound model over time. CT tracking revealed that while regulated negative pressure-assisted wound therapy reduced bacterial load to a limited extent (5%), RI-NPT significantly increased bacterial outflow and clearance (by 45%). This nanotechnology-based approach demonstrates the ability to track bacteria in vivo at real time and also the ability of the RI-NPT technology to effectively reduce bacterial load.",

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Sadan, T, Topaz, G, Popovtzer, R, Topaz, M & Fixler, D 2020, Tracking bacteria clearance using gold nanoparticles for wound therapy applications. in D Fixler, EM Goldys & S Wachsmann-Hogiu (eds), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII., 112541H, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11254, SPIE, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII 2020, San Francisco, United States, 2/02/20. https://doi.org/10.1117/12.2548441

Tracking bacteria clearance using gold nanoparticles for wound therapy applications. / Sadan, Tamar; Topaz, Guy; Popovtzer, Rachela et al.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII. ed. / Dror Fixler; Ewa M. Goldys; Sebastian Wachsmann-Hogiu. SPIE, 2020. 112541H (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11254).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Topaz, Guy

AU - Popovtzer, Rachela

AU - Topaz, Moris

AU - Fixler, Dror

N1 - Publisher Copyright: © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

PY - 2020

Y1 - 2020

N2 - Bacterial infection and biofilm buildup is a major challenge for wound healing. In the present work we developed a method to track bacteria in vivo in order to analyze the progress of the healing. Regulated irrigation combined with negative pressure (RI-NPT) is a nonpharmaceutical therapeutic strategy for reducing bacterial load in acute and chronically infected wounds. Here, we analyzed RI-NPT hydrokinetics and efficacy of bacterial load reduction in wounds. Escherichia coli were loaded with gold nanoparticles, in order to make them "visible" and trackable using computed tomography (CT). Bacteria were tracked using CT in a low-flow rat wound model over time. CT tracking revealed that while regulated negative pressure-assisted wound therapy reduced bacterial load to a limited extent (5%), RI-NPT significantly increased bacterial outflow and clearance (by 45%). This nanotechnology-based approach demonstrates the ability to track bacteria in vivo at real time and also the ability of the RI-NPT technology to effectively reduce bacterial load.

AB - Bacterial infection and biofilm buildup is a major challenge for wound healing. In the present work we developed a method to track bacteria in vivo in order to analyze the progress of the healing. Regulated irrigation combined with negative pressure (RI-NPT) is a nonpharmaceutical therapeutic strategy for reducing bacterial load in acute and chronically infected wounds. Here, we analyzed RI-NPT hydrokinetics and efficacy of bacterial load reduction in wounds. Escherichia coli were loaded with gold nanoparticles, in order to make them "visible" and trackable using computed tomography (CT). Bacteria were tracked using CT in a low-flow rat wound model over time. CT tracking revealed that while regulated negative pressure-assisted wound therapy reduced bacterial load to a limited extent (5%), RI-NPT significantly increased bacterial outflow and clearance (by 45%). This nanotechnology-based approach demonstrates the ability to track bacteria in vivo at real time and also the ability of the RI-NPT technology to effectively reduce bacterial load.

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A2 - Goldys, Ewa M.

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PB - SPIE

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Y2 - 2 February 2020 through 3 February 2020

ER -

Sadan T, Topaz G, Popovtzer R, Topaz M, Fixler D. Tracking bacteria clearance using gold nanoparticles for wound therapy applications. In Fixler D, Goldys EM, Wachsmann-Hogiu S, editors, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII. SPIE. 2020. 112541H. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2548441

Tracking bacteria clearance using gold nanoparticles for wound therapy applications

Abstract

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Keywords

All Science Journal Classification (ASJC) codes

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