Towards a consensus atlas of human and mouse adipose tissue at single-cell resolution

Anne Loft; Margo P. Emont; Ada Weinstock; Adeline Divoux; Adhideb Ghosh; Allon Wagner; Ann V. Hertzel; Babukrishna Maniyadath; Bart Deplancke; Boxiang Liu; Camilla Scheele; Carey Lumeng; Changhai Ding; Chenkai Ma; Christian Wolfrum; Clarissa Strieder-Barboza; Congru Li; Danh D. Truong; David A. Bernlohr; Elisabet Stener-Victorin; Erin E. Kershaw; Esti Yeger-Lotem; Farnaz Shamsi; Hannah X. Hui; Henrique Camara; Jiawei Zhong; Joanna Kalucka; Joseph A. Ludwig; Julie A. Semon; Jutta Jalkanen; Katie L. Whytock; Kyle D. Dumont; Lauren M. Sparks; Lindsey A. Muir; Lingzhao Fang; Lucas Massier; Luis R. Saraiva; Marc D. Beyer; Marc G. Jeschke; Marcelo A. Mori; Mariana Boroni; Martin J. Walsh; Mary Elizabeth Patti; Matthew D. Lynes; Matthias Blüher; Mikael Rydén; Natnael Hamda; Nicole L. Solimini; Niklas Mejhert; Peng Gao; Rana K. Gupta; Rinki Murphy; Saeed Pirouzpanah; Silvia Corvera; Su'an Tang; Swapan K. Das; Søren F. Schmidt; Tao Zhang; Theodore M. Nelson; Timothy E. O'Sullivan; Vissarion Efthymiou; Wenjing Wang; Yihan Tong; Yu Hua Tseng; Susanne Mandrup; Evan D. Rosen

doi:10.1038/s42255-025-01296-9

Towards a consensus atlas of human and mouse adipose tissue at single-cell resolution

Anne Loft, Margo P. Emont, Ada Weinstock, Adeline Divoux, Adhideb Ghosh, Allon Wagner, Ann V. Hertzel, Babukrishna Maniyadath, Bart Deplancke, Boxiang Liu, Camilla Scheele, Carey Lumeng, Changhai Ding, Chenkai Ma, Christian Wolfrum, Clarissa Strieder-Barboza, Congru Li, Danh D. Truong, David A. Bernlohr, Elisabet Stener-VictorinErin E. Kershaw, Esti Yeger-Lotem, Farnaz Shamsi, Hannah X. Hui, Henrique Camara, Jiawei Zhong, Joanna Kalucka, Joseph A. Ludwig, Julie A. Semon, Jutta Jalkanen, Katie L. Whytock, Kyle D. Dumont, Lauren M. Sparks, Lindsey A. Muir, Lingzhao Fang, Lucas Massier, Luis R. Saraiva, Marc D. Beyer, Marc G. Jeschke, Marcelo A. Mori, Mariana Boroni, Martin J. Walsh, Mary Elizabeth Patti, Matthew D. Lynes, Matthias Blüher, Mikael Rydén, Natnael Hamda, Nicole L. Solimini, Niklas Mejhert, Peng Gao, Rana K. Gupta, Rinki Murphy, Saeed Pirouzpanah, Silvia Corvera, Su'an Tang, Swapan K. Das, Søren F. Schmidt, Tao Zhang, Theodore M. Nelson, Timothy E. O'Sullivan, Vissarion Efthymiou, Wenjing Wang, Yihan Tong, Yu Hua Tseng, Susanne Mandrup, Evan D. Rosen

Department of Clinical Biochemistry and Pharmacology

Ben-Gurion University of the Negev

Research output: Contribution to journal › Review article › peer-review

Abstract

Adipose tissue (AT) is a complex connective tissue with a high relative proportion of adipocytes, which are specialized cells with the ability to store lipids in large droplets. AT is found in multiple discrete depots throughout the body, where it serves as the primary repository for excess calories. In addition, AT has an important role in functions as diverse as insulation, immunity and regulation of metabolic homeostasis. The Human Cell Atlas Adipose Bionetwork was established to support the generation of single-cell atlases of human AT as well as the development of unified approaches and consensus for cell annotation. Here, we provide a first roadmap from this bionetwork, including our suggested cell annotations for humans and mice, with the aim of describing the state of the field and providing guidelines for the production, analysis, interpretation and presentation of AT single-cell data.

Original language	American English
Pages (from-to)	875-894
Number of pages	20
Journal	Nature metabolism
Volume	7
Issue number	5
DOIs	https://doi.org/10.1038/s42255-025-01296-9
State	Published - 1 May 2025

All Science Journal Classification (ASJC) codes

Internal Medicine
Endocrinology, Diabetes and Metabolism
Physiology (medical)
Cell Biology

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10.1038/s42255-025-01296-9

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Loft, A., Emont, M. P., Weinstock, A., Divoux, A., Ghosh, A., Wagner, A., Hertzel, A. V., Maniyadath, B., Deplancke, B., Liu, B., Scheele, C., Lumeng, C., Ding, C., Ma, C., Wolfrum, C., Strieder-Barboza, C., Li, C., Truong, D. D., Bernlohr, D. A., ... Rosen, E. D. (2025). Towards a consensus atlas of human and mouse adipose tissue at single-cell resolution. Nature metabolism, 7(5), 875-894. https://doi.org/10.1038/s42255-025-01296-9

@article{f749aa32ba3e4a9dbafbd29407f3a164,

title = "Towards a consensus atlas of human and mouse adipose tissue at single-cell resolution",

abstract = "Adipose tissue (AT) is a complex connective tissue with a high relative proportion of adipocytes, which are specialized cells with the ability to store lipids in large droplets. AT is found in multiple discrete depots throughout the body, where it serves as the primary repository for excess calories. In addition, AT has an important role in functions as diverse as insulation, immunity and regulation of metabolic homeostasis. The Human Cell Atlas Adipose Bionetwork was established to support the generation of single-cell atlases of human AT as well as the development of unified approaches and consensus for cell annotation. Here, we provide a first roadmap from this bionetwork, including our suggested cell annotations for humans and mice, with the aim of describing the state of the field and providing guidelines for the production, analysis, interpretation and presentation of AT single-cell data.",

author = "Anne Loft and Emont, \{Margo P.\} and Ada Weinstock and Adeline Divoux and Adhideb Ghosh and Allon Wagner and Hertzel, \{Ann V.\} and Babukrishna Maniyadath and Bart Deplancke and Boxiang Liu and Camilla Scheele and Carey Lumeng and Changhai Ding and Chenkai Ma and Christian Wolfrum and Clarissa Strieder-Barboza and Congru Li and Truong, \{Danh D.\} and Bernlohr, \{David A.\} and Elisabet Stener-Victorin and Kershaw, \{Erin E.\} and Esti Yeger-Lotem and Farnaz Shamsi and Hui, \{Hannah X.\} and Henrique Camara and Jiawei Zhong and Joanna Kalucka and Ludwig, \{Joseph A.\} and Semon, \{Julie A.\} and Jutta Jalkanen and Whytock, \{Katie L.\} and Dumont, \{Kyle D.\} and Sparks, \{Lauren M.\} and Muir, \{Lindsey A.\} and Lingzhao Fang and Lucas Massier and Saraiva, \{Luis R.\} and Beyer, \{Marc D.\} and Jeschke, \{Marc G.\} and Mori, \{Marcelo A.\} and Mariana Boroni and Walsh, \{Martin J.\} and Patti, \{Mary Elizabeth\} and Lynes, \{Matthew D.\} and Matthias Bl{\"u}her and Mikael Ryd{\'e}n and Natnael Hamda and Solimini, \{Nicole L.\} and Niklas Mejhert and Peng Gao and Gupta, \{Rana K.\} and Rinki Murphy and Saeed Pirouzpanah and Silvia Corvera and Su'an Tang and Das, \{Swapan K.\} and Schmidt, \{S{\o}ren F.\} and Tao Zhang and Nelson, \{Theodore M.\} and O'Sullivan, \{Timothy E.\} and Vissarion Efthymiou and Wenjing Wang and Yihan Tong and Tseng, \{Yu Hua\} and Susanne Mandrup and Rosen, \{Evan D.\}",

note = "Publisher Copyright: {\textcopyright} 2025. Springer Nature Limited.",

year = "2025",

month = may,

day = "1",

doi = "10.1038/s42255-025-01296-9",

language = "American English",

volume = "7",

pages = "875--894",

journal = "Nature metabolism",

issn = "2522-5812",

publisher = "Springer Berlin",

number = "5",

}

Loft, A, Emont, MP, Weinstock, A, Divoux, A, Ghosh, A, Wagner, A, Hertzel, AV, Maniyadath, B, Deplancke, B, Liu, B, Scheele, C, Lumeng, C, Ding, C, Ma, C, Wolfrum, C, Strieder-Barboza, C, Li, C, Truong, DD, Bernlohr, DA, Stener-Victorin, E, Kershaw, EE, Yeger-Lotem, E, Shamsi, F, Hui, HX, Camara, H, Zhong, J, Kalucka, J, Ludwig, JA, Semon, JA, Jalkanen, J, Whytock, KL, Dumont, KD, Sparks, LM, Muir, LA, Fang, L, Massier, L, Saraiva, LR, Beyer, MD, Jeschke, MG, Mori, MA, Boroni, M, Walsh, MJ, Patti, ME, Lynes, MD, Blüher, M, Rydén, M, Hamda, N, Solimini, NL, Mejhert, N, Gao, P, Gupta, RK, Murphy, R, Pirouzpanah, S, Corvera, S, Tang, S, Das, SK, Schmidt, SF, Zhang, T, Nelson, TM, O'Sullivan, TE, Efthymiou, V, Wang, W, Tong, Y, Tseng, YH, Mandrup, S & Rosen, ED 2025, 'Towards a consensus atlas of human and mouse adipose tissue at single-cell resolution', Nature metabolism, vol. 7, no. 5, pp. 875-894. https://doi.org/10.1038/s42255-025-01296-9

TY - JOUR

T1 - Towards a consensus atlas of human and mouse adipose tissue at single-cell resolution

AU - Loft, Anne

AU - Emont, Margo P.

AU - Weinstock, Ada

AU - Divoux, Adeline

AU - Ghosh, Adhideb

AU - Wagner, Allon

AU - Hertzel, Ann V.

AU - Maniyadath, Babukrishna

AU - Deplancke, Bart

AU - Liu, Boxiang

AU - Scheele, Camilla

AU - Lumeng, Carey

AU - Ding, Changhai

AU - Ma, Chenkai

AU - Wolfrum, Christian

AU - Strieder-Barboza, Clarissa

AU - Li, Congru

AU - Truong, Danh D.

AU - Bernlohr, David A.

AU - Stener-Victorin, Elisabet

AU - Kershaw, Erin E.

AU - Yeger-Lotem, Esti

AU - Shamsi, Farnaz

AU - Hui, Hannah X.

AU - Camara, Henrique

AU - Zhong, Jiawei

AU - Kalucka, Joanna

AU - Ludwig, Joseph A.

AU - Semon, Julie A.

AU - Jalkanen, Jutta

AU - Whytock, Katie L.

AU - Dumont, Kyle D.

AU - Sparks, Lauren M.

AU - Muir, Lindsey A.

AU - Fang, Lingzhao

AU - Massier, Lucas

AU - Saraiva, Luis R.

AU - Beyer, Marc D.

AU - Jeschke, Marc G.

AU - Mori, Marcelo A.

AU - Boroni, Mariana

AU - Walsh, Martin J.

AU - Patti, Mary Elizabeth

AU - Lynes, Matthew D.

AU - Blüher, Matthias

AU - Rydén, Mikael

AU - Hamda, Natnael

AU - Solimini, Nicole L.

AU - Mejhert, Niklas

AU - Gao, Peng

AU - Gupta, Rana K.

AU - Murphy, Rinki

AU - Pirouzpanah, Saeed

AU - Corvera, Silvia

AU - Tang, Su'an

AU - Das, Swapan K.

AU - Schmidt, Søren F.

AU - Zhang, Tao

AU - Nelson, Theodore M.

AU - O'Sullivan, Timothy E.

AU - Efthymiou, Vissarion

AU - Wang, Wenjing

AU - Tong, Yihan

AU - Tseng, Yu Hua

AU - Mandrup, Susanne

AU - Rosen, Evan D.

PY - 2025/5/1

Y1 - 2025/5/1

N2 - Adipose tissue (AT) is a complex connective tissue with a high relative proportion of adipocytes, which are specialized cells with the ability to store lipids in large droplets. AT is found in multiple discrete depots throughout the body, where it serves as the primary repository for excess calories. In addition, AT has an important role in functions as diverse as insulation, immunity and regulation of metabolic homeostasis. The Human Cell Atlas Adipose Bionetwork was established to support the generation of single-cell atlases of human AT as well as the development of unified approaches and consensus for cell annotation. Here, we provide a first roadmap from this bionetwork, including our suggested cell annotations for humans and mice, with the aim of describing the state of the field and providing guidelines for the production, analysis, interpretation and presentation of AT single-cell data.

AB - Adipose tissue (AT) is a complex connective tissue with a high relative proportion of adipocytes, which are specialized cells with the ability to store lipids in large droplets. AT is found in multiple discrete depots throughout the body, where it serves as the primary repository for excess calories. In addition, AT has an important role in functions as diverse as insulation, immunity and regulation of metabolic homeostasis. The Human Cell Atlas Adipose Bionetwork was established to support the generation of single-cell atlases of human AT as well as the development of unified approaches and consensus for cell annotation. Here, we provide a first roadmap from this bionetwork, including our suggested cell annotations for humans and mice, with the aim of describing the state of the field and providing guidelines for the production, analysis, interpretation and presentation of AT single-cell data.

UR - http://www.scopus.com/inward/record.url?scp=105007119898&partnerID=8YFLogxK

U2 - 10.1038/s42255-025-01296-9

DO - 10.1038/s42255-025-01296-9

M3 - Review article

C2 - 40360756

SN - 2522-5812

VL - 7

SP - 875

EP - 894

JO - Nature metabolism

JF - Nature metabolism

IS - 5

ER -

Towards a consensus atlas of human and mouse adipose tissue at single-cell resolution

Abstract

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