TY - JOUR
T1 - Toward dynamic structural biology
T2 - Two decades of single-molecule förster resonance energy transfer
AU - Lerner, Eitan
AU - Cordes, Thorben
AU - Ingargiola, Antonino
AU - Alhadid, Yazan
AU - Chung, Sang Yoon
AU - Michalet, Xavier
AU - Weiss, Shimon
N1 - Funding Information: We acknowledge contributions and discussions with past and present members of the Weiss lab. We thank M. Segal for editing the manuscript. Supported by NIH grants GM069709 and NSF grant MCB-1244175 (S.W.), NIH grant GM095904 (X.M. and S.W.), and European Research Council grant ERC-STG 638536–SM-IMPORT, grant of the Deutsche Forschungsgemeinschaft within GRK2062, the Center for NanoScience, Center for Integrated Protein Science Munich, and LMUexcellent (T.C.).
PY - 2018/1/19
Y1 - 2018/1/19
N2 - Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Förster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and provided new insights into many fundamental biological processes, such as DNA maintenance and repair, transcription, translation, and membrane transport. We review 22 years of contributions of smFRET to our understanding of basic mechanisms in biochemistry, molecular biology, and structural biology. Additionally, building on current state-of-the-art implementations of smFRET, we highlight possible future directions for smFRET in applications such as biosensing, high-throughput screening, and molecular diagnostics.
AB - Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Förster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and provided new insights into many fundamental biological processes, such as DNA maintenance and repair, transcription, translation, and membrane transport. We review 22 years of contributions of smFRET to our understanding of basic mechanisms in biochemistry, molecular biology, and structural biology. Additionally, building on current state-of-the-art implementations of smFRET, we highlight possible future directions for smFRET in applications such as biosensing, high-throughput screening, and molecular diagnostics.
UR - http://www.scopus.com/inward/record.url?scp=85040792381&partnerID=8YFLogxK
U2 - 10.1126/science.aan1133
DO - 10.1126/science.aan1133
M3 - مقالة مرجعية
C2 - 29348210
SN - 0036-8075
VL - 359
JO - Science
JF - Science
IS - 6373
M1 - eaan1133
ER -