TY - CHAP
T1 - To set or not to set
T2 - New tools to study the control of cucumber fruit set
AU - Shnaider, Y.
AU - Brotman, Y.
AU - Perl-Treves, R.
PY - 2017/3/20
Y1 - 2017/3/20
N2 - Understanding the developmental control of fruit set is an important challenge. In cucumber, fruit set is governed by several hormones, including gibberellin, cytokinin and auxin, and is also affected by assimilate supply and previous fruit load. We have produced transgenic cucumbers that express reporter genes under the control of the Exp, ARR5 and DR5 promoters, reported to respond to gibberellin, cytokinin and auxin, respectively. Specific expression patterns were observed in ovaries and ovules of cucumber at different stages, and we try to correlate these with ovary developmental fates. To determine the possible role of metabolite supply in fruit set, we monitored the transcript levels of sugar-metabolism genes in the ovaries, including ovary-specific invertases, as well as a fruit-set related galactosyl transferase. Finally, metabolomic analysis of ovaries has been initiated, to look at metabolic changes that mark cucumber fruit set.
AB - Understanding the developmental control of fruit set is an important challenge. In cucumber, fruit set is governed by several hormones, including gibberellin, cytokinin and auxin, and is also affected by assimilate supply and previous fruit load. We have produced transgenic cucumbers that express reporter genes under the control of the Exp, ARR5 and DR5 promoters, reported to respond to gibberellin, cytokinin and auxin, respectively. Specific expression patterns were observed in ovaries and ovules of cucumber at different stages, and we try to correlate these with ovary developmental fates. To determine the possible role of metabolite supply in fruit set, we monitored the transcript levels of sugar-metabolism genes in the ovaries, including ovary-specific invertases, as well as a fruit-set related galactosyl transferase. Finally, metabolomic analysis of ovaries has been initiated, to look at metabolic changes that mark cucumber fruit set.
KW - Cucumber
KW - Fruit set
KW - Hormone reporter genes
KW - Metabolomics
UR - http://www.scopus.com/inward/record.url?scp=85017530818&partnerID=8YFLogxK
U2 - https://doi.org/10.17660/ActaHortic.2017.1151.28
DO - https://doi.org/10.17660/ActaHortic.2017.1151.28
M3 - فصل
T3 - Acta Horticulturae
SP - 175
EP - 182
BT - 5th International Symposium on Cucurbits
A2 - Fernandez, J.A.
A2 - Alfocea, F. Perez
A2 - Gomez-Guillamon, M.L.
PB - International Society for Horticultural Science
ER -