TY - JOUR
T1 - Tissue topography steers migrating Drosophila border cells
AU - Dai, Wei
AU - Guo, Xiaoran
AU - Cao, Yuansheng
AU - Mondo, James A.
AU - Campanale, Joseph P.
AU - Montell, Brandon J.
AU - Burrous, Haley
AU - Streichan, Sebastian
AU - Gov, Nir
AU - Rappel, Wouter Jan
AU - Montell, Denise J.
N1 - We thank B. Cheng, S. Dhar, Y. Li, M. A. Pastor, and R. Wu for technical assistance. Funding: This work was supported by NIH grant GM46425 to D.J.M., NSF grant PHY-1707637 to W.-J.R., and ACS grant PF-17-024-01-CSM to J.P.C. We thank the Developmental Studies Hybridoma Bank for providing antibodies and H. Sung, H. J. Rene, the Bloomington Drosophila Stock Center, and the Vienna Drosophila Resource Center for providing fly stocks. We acknowledge the use of the NRI-MCDB Microscopy Facility and the Imaris computer workstation supported by the Office of the Director, NIH, under award no. S10OD010610. N.S.G. is the incumbent of the Lee and William Abramowitz Professorial Chair of Biophysics and this research was supported by the Israel Science Foundation (grant no. 1459/17). Author contributions: W.D., X.G., J.A.M., J.P.C., and D.J.M. designed experiments. W.D., X.G., J.A.M., J.P.C., and H.B. performed experiments. Y.C., W.-J.R., and N.G. performed modeling. B.J.M. produced graphical illustrations and animations. S.S. assisted with light sheet imaging and data analysis. W.D., X.G., Y.C., J.A.M., J.P.C., N.G., W.-J.R., and D.J.M. prepared the manuscript.
PY - 2020/11/20
Y1 - 2020/11/20
N2 - Moving cells can sense and respond to physical features of the microenvironment; however, in vivo, the significance of tissue topography is mostly unknown. Here, we used Drosophila border cells, an established model for in vivo cell migration, to study how chemical and physical information influences path selection. Although chemical cues were thought to be sufficient, live imaging, genetics, modeling, and simulations show that microtopography is also important. Chemoattractants promote predominantly posterior movement, whereas tissue architecture presents orthogonal information, a path of least resistance concentrated near the center of the egg chamber. E-cadherin supplies a permissive haptotactic cue. Our results provide insight into how cells integrate and prioritize topographical, adhesive, and chemoattractant cues to choose one path among many.
AB - Moving cells can sense and respond to physical features of the microenvironment; however, in vivo, the significance of tissue topography is mostly unknown. Here, we used Drosophila border cells, an established model for in vivo cell migration, to study how chemical and physical information influences path selection. Although chemical cues were thought to be sufficient, live imaging, genetics, modeling, and simulations show that microtopography is also important. Chemoattractants promote predominantly posterior movement, whereas tissue architecture presents orthogonal information, a path of least resistance concentrated near the center of the egg chamber. E-cadherin supplies a permissive haptotactic cue. Our results provide insight into how cells integrate and prioritize topographical, adhesive, and chemoattractant cues to choose one path among many.
UR - http://www.scopus.com/inward/record.url?scp=85096458884&partnerID=8YFLogxK
U2 - 10.1126/science.aaz4741
DO - 10.1126/science.aaz4741
M3 - مقالة
C2 - 33214282
SN - 0036-8075
VL - 370
SP - 987
EP - 990
JO - Science (New York, N.Y.)
JF - Science (New York, N.Y.)
IS - 6519
ER -