Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis

Elle Koren; Alona Feldman; Marianna Yusupova; Avihay Kadosh; Egor Sedov; Roi Ankawa; Yahav Yosefzon; Waseem Nasser; Stefanie Gerstberger; Liam B. Kimel; Noa Priselac; Samara Brown; Sam Sharma; Travis Gorenc; Ruby Shalom-Feuerstein; Hermann Steller; Tom Shemesh; Yaron Fuchs

doi:https://doi.org/10.1038/s41467-022-31629-1

Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis

Elle Koren, Alona Feldman, Marianna Yusupova, Avihay Kadosh, Egor Sedov, Roi Ankawa, Yahav Yosefzon, Waseem Nasser, Stefanie Gerstberger, Liam B. Kimel, Noa Priselac, Samara Brown, Sam Sharma, Travis Gorenc, Ruby Shalom-Feuerstein, Hermann Steller, Tom Shemesh, Yaron Fuchs

Technion - Israel Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

The presence of distinct stem cells that maintain the interfollicular epidermis is highly debated. Here, we report a population of keratinocytes, marked by Thy1, in the basal layer of the interfollicular epidermis. We find that epidermal cells expressing differential levels of Thy1 display distinct transcriptional signatures. Thy1⁺ keratinocytes do not express T cell markers, express a unique transcriptional profile, cycle significantly slower than basal epidermal progenitors and display significant expansion potential in vitro. Multicolor lineage tracing analyses and mathematical modeling reveal that Thy1⁺ basal keratinocytes do not compete neutrally alike interfollicular progenitors and contribute long-term to both epidermal replenishment and wound repair. Importantly, ablation of Thy1⁺ cells strongly impairs these processes, thus indicating the non-redundant function of Thy1⁺ stem cells in the epidermis. Collectively, these results reveal a distinct stem cell population that plays a critical role in epidermal homeostasis and repair.

Original language	English
Article number	4628
Pages (from-to)	4628
Journal	Nature Communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-31629-1
State	Published - 8 Aug 2022

Keywords

Animals
Cell Differentiation/physiology
Epidermal Cells
Epidermis/metabolism
Keratinocytes/metabolism
Mice
Stem Cells/metabolism

All Science Journal Classification (ASJC) codes

General Chemistry
General
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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https://doi.org/10.1038/s41467-022-31629-1

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Koren, E., Feldman, A., Yusupova, M., Kadosh, A., Sedov, E., Ankawa, R., Yosefzon, Y., Nasser, W., Gerstberger, S., Kimel, L. B., Priselac, N., Brown, S., Sharma, S., Gorenc, T., Shalom-Feuerstein, R., Steller, H., Shemesh, T., & Fuchs, Y. (2022). Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis. Nature Communications, 13(1), 4628. Article 4628. https://doi.org/10.1038/s41467-022-31629-1

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title = "Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis",

abstract = "The presence of distinct stem cells that maintain the interfollicular epidermis is highly debated. Here, we report a population of keratinocytes, marked by Thy1, in the basal layer of the interfollicular epidermis. We find that epidermal cells expressing differential levels of Thy1 display distinct transcriptional signatures. Thy1+ keratinocytes do not express T cell markers, express a unique transcriptional profile, cycle significantly slower than basal epidermal progenitors and display significant expansion potential in vitro. Multicolor lineage tracing analyses and mathematical modeling reveal that Thy1+ basal keratinocytes do not compete neutrally alike interfollicular progenitors and contribute long-term to both epidermal replenishment and wound repair. Importantly, ablation of Thy1+ cells strongly impairs these processes, thus indicating the non-redundant function of Thy1+ stem cells in the epidermis. Collectively, these results reveal a distinct stem cell population that plays a critical role in epidermal homeostasis and repair.",

keywords = "Animals, Cell Differentiation/physiology, Epidermal Cells, Epidermis/metabolism, Keratinocytes/metabolism, Mice, Stem Cells/metabolism",

author = "Elle Koren and Alona Feldman and Marianna Yusupova and Avihay Kadosh and Egor Sedov and Roi Ankawa and Yahav Yosefzon and Waseem Nasser and Stefanie Gerstberger and Kimel, {Liam B.} and Noa Priselac and Samara Brown and Sam Sharma and Travis Gorenc and Ruby Shalom-Feuerstein and Hermann Steller and Tom Shemesh and Yaron Fuchs",

note = "{\textcopyright} 2022. The Author(s).",

year = "2022",

month = aug,

day = "8",

doi = "https://doi.org/10.1038/s41467-022-31629-1",

language = "الإنجليزيّة",

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pages = "4628",

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Koren, E, Feldman, A, Yusupova, M, Kadosh, A, Sedov, E, Ankawa, R, Yosefzon, Y, Nasser, W, Gerstberger, S, Kimel, LB, Priselac, N, Brown, S, Sharma, S, Gorenc, T, Shalom-Feuerstein, R, Steller, H, Shemesh, T & Fuchs, Y 2022, 'Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis', Nature Communications, vol. 13, no. 1, 4628, pp. 4628. https://doi.org/10.1038/s41467-022-31629-1

TY - JOUR

T1 - Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis

AU - Koren, Elle

AU - Feldman, Alona

AU - Yusupova, Marianna

AU - Kadosh, Avihay

AU - Sedov, Egor

AU - Ankawa, Roi

AU - Yosefzon, Yahav

AU - Nasser, Waseem

AU - Gerstberger, Stefanie

AU - Kimel, Liam B.

AU - Priselac, Noa

AU - Brown, Samara

AU - Sharma, Sam

AU - Gorenc, Travis

AU - Shalom-Feuerstein, Ruby

AU - Steller, Hermann

AU - Shemesh, Tom

AU - Fuchs, Yaron

PY - 2022/8/8

Y1 - 2022/8/8

N2 - The presence of distinct stem cells that maintain the interfollicular epidermis is highly debated. Here, we report a population of keratinocytes, marked by Thy1, in the basal layer of the interfollicular epidermis. We find that epidermal cells expressing differential levels of Thy1 display distinct transcriptional signatures. Thy1+ keratinocytes do not express T cell markers, express a unique transcriptional profile, cycle significantly slower than basal epidermal progenitors and display significant expansion potential in vitro. Multicolor lineage tracing analyses and mathematical modeling reveal that Thy1+ basal keratinocytes do not compete neutrally alike interfollicular progenitors and contribute long-term to both epidermal replenishment and wound repair. Importantly, ablation of Thy1+ cells strongly impairs these processes, thus indicating the non-redundant function of Thy1+ stem cells in the epidermis. Collectively, these results reveal a distinct stem cell population that plays a critical role in epidermal homeostasis and repair.

AB - The presence of distinct stem cells that maintain the interfollicular epidermis is highly debated. Here, we report a population of keratinocytes, marked by Thy1, in the basal layer of the interfollicular epidermis. We find that epidermal cells expressing differential levels of Thy1 display distinct transcriptional signatures. Thy1+ keratinocytes do not express T cell markers, express a unique transcriptional profile, cycle significantly slower than basal epidermal progenitors and display significant expansion potential in vitro. Multicolor lineage tracing analyses and mathematical modeling reveal that Thy1+ basal keratinocytes do not compete neutrally alike interfollicular progenitors and contribute long-term to both epidermal replenishment and wound repair. Importantly, ablation of Thy1+ cells strongly impairs these processes, thus indicating the non-redundant function of Thy1+ stem cells in the epidermis. Collectively, these results reveal a distinct stem cell population that plays a critical role in epidermal homeostasis and repair.

KW - Animals

KW - Cell Differentiation/physiology

KW - Epidermal Cells

KW - Epidermis/metabolism

KW - Keratinocytes/metabolism

KW - Mice

KW - Stem Cells/metabolism

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U2 - https://doi.org/10.1038/s41467-022-31629-1

DO - https://doi.org/10.1038/s41467-022-31629-1

M3 - مقالة

C2 - 35941116

SN - 2041-1723

VL - 13

SP - 4628

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 4628

ER -

Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis

Abstract

Keywords

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