Three-dimensional imaging using phase retrieval with two focus planes

Tali Ilovitsh, Asaf Ilovitsh, Aryeh Weiss, Rinat Meir, Zeev Zalevsky

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.

Original languageEnglish
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XXIII
EditorsCarol J. Cogswell, Tony Wilson, Thomas G. Brown
ISBN (Electronic)9781628419474
StatePublished - 2016
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII - San Francisco, United States
Duration: 15 Feb 201617 Feb 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE


ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIII
Country/TerritoryUnited States
CitySan Francisco


  • Cellular imaging
  • Phase retrieval
  • Three-dimensional microscopy
  • gold nanoparticle

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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