The use of gapmers for in vivo suppression of hepatic mRNA targets

David S. Greenberg; Yonat Tzur; Hermona Soreq

doi:https://doi.org/10.1007/978-1-0716-0771-8_13

The use of gapmers for in vivo suppression of hepatic mRNA targets

David S. Greenberg, Yonat Tzur, Hermona Soreq

The Hebrew University of Jerusalem

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

This chapter describes the use of locked nucleic acid (LNA) GapmeRs for the in vivo knockdown of specific mRNAs in the mouse liver and phenotype analysis. LNA GapmeRs may be tested for efficacy by transfection in cultured cells. They are delivered into mice in vivo by intravenous tail injection.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	177-184
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-0716-0771-8_13
State	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2176

Keywords

Diet-induced obese mice
In vivo knockdown
Liver mRNA
LNA gapmers
Steatosis

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

https://doi.org/10.1007/978-1-0716-0771-8_13

Cite this

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abstract = "This chapter describes the use of locked nucleic acid (LNA) GapmeRs for the in vivo knockdown of specific mRNAs in the mouse liver and phenotype analysis. LNA GapmeRs may be tested for efficacy by transfection in cultured cells. They are delivered into mice in vivo by intravenous tail injection.",

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Y1 - 2020

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KW - In vivo knockdown

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KW - LNA gapmers

KW - Steatosis

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The use of gapmers for in vivo suppression of hepatic mRNA targets

Abstract

Publication series

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

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Cite this