The use of EPR spectroscopy to study transcription mechanisms

L. Hofmann, A. Mandato, S. Saxena, S. Ruthstein

Research output: Contribution to journalReview articlepeer-review

Abstract

Electron paramagnetic resonance (EPR) spectroscopy has become a promising structural biology tool to resolve complex and dynamic biological mechanisms in-vitro and in-cell. Here, we focus on the advantages of continuous wave (CW) and pulsed EPR distance measurements to resolve transcription processes and protein-DNA interaction. The wide range of spin-labeling approaches that can be used to follow structural changes in both protein and DNA render EPR a powerful method to study protein-DNA interactions and structure–function relationships in other macromolecular complexes. EPR-derived data goes well beyond static structural information and thus serves as the method of choice if dynamic insight is needed. Herein, we describe the conceptual details of the theory and the methodology and illustrate the use of EPR to study the protein-DNA interaction of the copper-sensitive transcription factor, CueR.

Original languageEnglish
Pages (from-to)1141-1159
Number of pages19
JournalBiophysical Reviews
Volume14
Issue number5
DOIs
StatePublished - Oct 2022

Keywords

  • CW-EPR
  • CueR
  • DEER
  • EPR spectroscopy
  • Protein-DNA interaction

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biophysics
  • Structural Biology

Fingerprint

Dive into the research topics of 'The use of EPR spectroscopy to study transcription mechanisms'. Together they form a unique fingerprint.

Cite this