TY - JOUR
T1 - The unravelling of the ubiquitin system
AU - Ciechanover, Aaron
N1 - Funding Information: Research in the author’s laboratory is supported by grants from the Dr. Miriam and Sheldon G. Adelson Medical Research Foundation (AMRF), the Israel Science Foundation (ISF), the Israeli Centers for Research Excellence (I-CORE) Program of the Israeli Planning and Budgeting Committee and the Israel Science Foundation (ISF) (Grant1775/12), the EU Treat PolyQ Network, and the Deutsch-Israelische Projektkooperation (DIP). The author is an Israel Cancer Research Fund (ICRF) USA professor. Publisher Copyright: © 2015 Macmillan Publishers Limited. All rights reserved.
PY - 2015/4/23
Y1 - 2015/4/23
N2 - Today, many scientific discoveries are made using a top-down experimental approach. The ubiquitin system was discovered using a 'classic' bottom-up approach to tackle the question: 'how are cellular proteins selectively degraded?' A simple proteolytic assay, which used a crude cell-extract, was all that was required to address this question; it was followed by fractionation and reconstitution experiments to decipher the role of the components in this multi-step process. This 'biochemistry at its best' approach, which was published in a periodical that today would not be regarded as highly visible, provided magnificent findings.
AB - Today, many scientific discoveries are made using a top-down experimental approach. The ubiquitin system was discovered using a 'classic' bottom-up approach to tackle the question: 'how are cellular proteins selectively degraded?' A simple proteolytic assay, which used a crude cell-extract, was all that was required to address this question; it was followed by fractionation and reconstitution experiments to decipher the role of the components in this multi-step process. This 'biochemistry at its best' approach, which was published in a periodical that today would not be regarded as highly visible, provided magnificent findings.
UR - https://www.scopus.com/pages/publications/84929086199
U2 - 10.1038/nrm3982
DO - 10.1038/nrm3982
M3 - Review article
SN - 1471-0072
VL - 16
SP - 322
EP - 324
JO - Nature Reviews Molecular Cell Biology
JF - Nature Reviews Molecular Cell Biology
IS - 5
ER -