The underappreciated diversity of bile acid modifications

Ipsita Mohanty; Helena Mannochio-Russo; Joshua V. Schweer; Yasin El Abiead; Wout Bittremieux; Shipei Xing; Robin Schmid; Simone Zuffa; Felipe Vasquez; Valentina B. Muti; Jasmine Zemlin; Omar E. Tovar-Herrera; Sarah Moraïs; Dhimant Desai; Shantu Amin; Imhoi Koo; Christoph W. Turck; Itzhak Mizrahi; Penny M. Kris-Etherton; Kristina S. Petersen; Jennifer A. Fleming; Tao Huan; Andrew D. Patterson; Dionicio Siegel; Lee R. Hagey; Mingxun Wang; Allegra T. Aron; Pieter C. Dorrestein

doi:10.1016/j.cell.2024.02.019

The underappreciated diversity of bile acid modifications

Ipsita Mohanty, Helena Mannochio-Russo, Joshua V. Schweer, Yasin El Abiead, Wout Bittremieux, Shipei Xing, Robin Schmid, Simone Zuffa, Felipe Vasquez, Valentina B. Muti, Jasmine Zemlin, Omar E. Tovar-Herrera, Sarah Moraïs, Dhimant Desai, Shantu Amin, Imhoi Koo, Christoph W. Turck, Itzhak Mizrahi, Penny M. Kris-Etherton, Kristina S. PetersenJennifer A. Fleming, Tao Huan, Andrew D. Patterson, Dionicio Siegel, Lee R. Hagey, Mingxun Wang, Allegra T. Aron, Pieter C. Dorrestein

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

The repertoire of modifications to bile acids and related steroidal lipids by host and microbial metabolism remains incompletely characterized. To address this knowledge gap, we created a reusable resource of tandem mass spectrometry (MS/MS) spectra by filtering 1.2 billion publicly available MS/MS spectra for bile-acid-selective ion patterns. Thousands of modifications are distributed throughout animal and human bodies as well as microbial cultures. We employed this MS/MS library to identify polyamine bile amidates, prevalent in carnivores. They are present in humans, and their levels alter with a diet change from a Mediterranean to a typical American diet. This work highlights the existence of many more bile acid modifications than previously recognized and the value of leveraging public large-scale untargeted metabolomics data to discover metabolites. The availability of a modification-centric bile acid MS/MS library will inform future studies investigating bile acid roles in health and disease.

Original language	American English
Pages (from-to)	1801-1818.e20
Journal	Cell
Volume	187
Issue number	7
DOIs	https://doi.org/10.1016/j.cell.2024.02.019
State	Published - 28 Mar 2024

Keywords

GABA
MassQL
agmatine
bile acids
diet
fastMASST
microbial
polyamines
putrescine
spectral resource

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

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10.1016/j.cell.2024.02.019

Cite this

Mohanty, I., Mannochio-Russo, H., Schweer, J. V., El Abiead, Y., Bittremieux, W., Xing, S., Schmid, R., Zuffa, S., Vasquez, F., Muti, V. B., Zemlin, J., Tovar-Herrera, O. E., Moraïs, S., Desai, D., Amin, S., Koo, I., Turck, C. W., Mizrahi, I., Kris-Etherton, P. M., ... Dorrestein, P. C. (2024). The underappreciated diversity of bile acid modifications. Cell, 187(7), 1801-1818.e20. https://doi.org/10.1016/j.cell.2024.02.019

@article{da6bbb18998d4efa930fcb2873ea4489,

title = "The underappreciated diversity of bile acid modifications",

abstract = "The repertoire of modifications to bile acids and related steroidal lipids by host and microbial metabolism remains incompletely characterized. To address this knowledge gap, we created a reusable resource of tandem mass spectrometry (MS/MS) spectra by filtering 1.2 billion publicly available MS/MS spectra for bile-acid-selective ion patterns. Thousands of modifications are distributed throughout animal and human bodies as well as microbial cultures. We employed this MS/MS library to identify polyamine bile amidates, prevalent in carnivores. They are present in humans, and their levels alter with a diet change from a Mediterranean to a typical American diet. This work highlights the existence of many more bile acid modifications than previously recognized and the value of leveraging public large-scale untargeted metabolomics data to discover metabolites. The availability of a modification-centric bile acid MS/MS library will inform future studies investigating bile acid roles in health and disease.",

keywords = "GABA, MassQL, agmatine, bile acids, diet, fastMASST, microbial, polyamines, putrescine, spectral resource",

author = "Ipsita Mohanty and Helena Mannochio-Russo and Schweer, \{Joshua V.\} and \{El Abiead\}, Yasin and Wout Bittremieux and Shipei Xing and Robin Schmid and Simone Zuffa and Felipe Vasquez and Muti, \{Valentina B.\} and Jasmine Zemlin and Tovar-Herrera, \{Omar E.\} and Sarah Mora{\"i}s and Dhimant Desai and Shantu Amin and Imhoi Koo and Turck, \{Christoph W.\} and Itzhak Mizrahi and Kris-Etherton, \{Penny M.\} and Petersen, \{Kristina S.\} and Fleming, \{Jennifer A.\} and Tao Huan and Patterson, \{Andrew D.\} and Dionicio Siegel and Hagey, \{Lee R.\} and Mingxun Wang and Aron, \{Allegra T.\} and Dorrestein, \{Pieter C.\}",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors",

year = "2024",

month = mar,

day = "28",

doi = "10.1016/j.cell.2024.02.019",

language = "American English",

volume = "187",

pages = "1801--1818.e20",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

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Mohanty, I, Mannochio-Russo, H, Schweer, JV, El Abiead, Y, Bittremieux, W, Xing, S, Schmid, R, Zuffa, S, Vasquez, F, Muti, VB, Zemlin, J, Tovar-Herrera, OE, Moraïs, S, Desai, D, Amin, S, Koo, I, Turck, CW, Mizrahi, I, Kris-Etherton, PM, Petersen, KS, Fleming, JA, Huan, T, Patterson, AD, Siegel, D, Hagey, LR, Wang, M, Aron, AT & Dorrestein, PC 2024, 'The underappreciated diversity of bile acid modifications', Cell, vol. 187, no. 7, pp. 1801-1818.e20. https://doi.org/10.1016/j.cell.2024.02.019

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T1 - The underappreciated diversity of bile acid modifications

AU - Mohanty, Ipsita

AU - Mannochio-Russo, Helena

AU - Schweer, Joshua V.

AU - El Abiead, Yasin

AU - Bittremieux, Wout

AU - Xing, Shipei

AU - Schmid, Robin

AU - Zuffa, Simone

AU - Vasquez, Felipe

AU - Muti, Valentina B.

AU - Zemlin, Jasmine

AU - Tovar-Herrera, Omar E.

AU - Moraïs, Sarah

AU - Desai, Dhimant

AU - Amin, Shantu

AU - Koo, Imhoi

AU - Turck, Christoph W.

AU - Mizrahi, Itzhak

AU - Kris-Etherton, Penny M.

AU - Petersen, Kristina S.

AU - Fleming, Jennifer A.

AU - Huan, Tao

AU - Patterson, Andrew D.

AU - Siegel, Dionicio

AU - Hagey, Lee R.

AU - Wang, Mingxun

AU - Aron, Allegra T.

AU - Dorrestein, Pieter C.

PY - 2024/3/28

Y1 - 2024/3/28

N2 - The repertoire of modifications to bile acids and related steroidal lipids by host and microbial metabolism remains incompletely characterized. To address this knowledge gap, we created a reusable resource of tandem mass spectrometry (MS/MS) spectra by filtering 1.2 billion publicly available MS/MS spectra for bile-acid-selective ion patterns. Thousands of modifications are distributed throughout animal and human bodies as well as microbial cultures. We employed this MS/MS library to identify polyamine bile amidates, prevalent in carnivores. They are present in humans, and their levels alter with a diet change from a Mediterranean to a typical American diet. This work highlights the existence of many more bile acid modifications than previously recognized and the value of leveraging public large-scale untargeted metabolomics data to discover metabolites. The availability of a modification-centric bile acid MS/MS library will inform future studies investigating bile acid roles in health and disease.

AB - The repertoire of modifications to bile acids and related steroidal lipids by host and microbial metabolism remains incompletely characterized. To address this knowledge gap, we created a reusable resource of tandem mass spectrometry (MS/MS) spectra by filtering 1.2 billion publicly available MS/MS spectra for bile-acid-selective ion patterns. Thousands of modifications are distributed throughout animal and human bodies as well as microbial cultures. We employed this MS/MS library to identify polyamine bile amidates, prevalent in carnivores. They are present in humans, and their levels alter with a diet change from a Mediterranean to a typical American diet. This work highlights the existence of many more bile acid modifications than previously recognized and the value of leveraging public large-scale untargeted metabolomics data to discover metabolites. The availability of a modification-centric bile acid MS/MS library will inform future studies investigating bile acid roles in health and disease.

KW - GABA

KW - MassQL

KW - agmatine

KW - bile acids

KW - diet

KW - fastMASST

KW - microbial

KW - polyamines

KW - putrescine

KW - spectral resource

UR - http://www.scopus.com/inward/record.url?scp=85188521465&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2024.02.019

DO - 10.1016/j.cell.2024.02.019

M3 - Article

C2 - 38471500

SN - 0092-8674

VL - 187

SP - 1801-1818.e20

JO - Cell

JF - Cell

IS - 7

ER -

The underappreciated diversity of bile acid modifications

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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