@article{b593434614a648c9a4d308f4146fc237,
title = "The substructure of three repetitive DNA regions of Schistosoma haematobium group species as a potential marker for species recognition and interbreeding detection",
abstract = "Background: Schistosoma haematobium is the causative agent of human urogenital schistosomiasis affecting ~112 million people in Africa and the Middle East. The parasite is transmitted by snails of the genus Bulinus, which also transmit other closely related human and animal schistosomes. The accurate discrimination of S. haematobium from species infecting animals will aid effective control and elimination programs. Previously we have shown the utility of different repetitive nuclear DNA sequences (DraI, sh73bp, and sh77bp) for the identification of S. haematobium-group species and inter-repeat sequences for discriminating S. haematobium from S. bovis. Results: In this current study we clarify the structural arrangement and association between the three repetitive sequences (DraI, sh73bp, and sh77bp) in both S. haematobium and S. bovis, with a unique repeat linker being found in S. haematobium (Sh64bp repeat linker) and in S. bovis (Sb30bp repeat linker). Sequence data showed that the 3′-end of the repeat linker was connected to the DraI repetitive sequence array, and at the 5′-end of the repeat linker sh73bp and sh77bp were arranged in an alternating manner. Species-specific oligonucleotides were designed targeting the species-specific repeat linkers and used in a reverse line blot (RLB) hybridization assay enabling differentiation between S. haematobium and S. bovis. The assay was used to discriminate natural infections in wild caught Bulinus globosus. Conclusion: This research enabled the characterisation of species-specific DNA regions that enabled the design of species-specific oligonucleotides that can be used to rapidly differentiate between S. haematobium and S. bovis and also have the potential to aid the detection of natural hybridization between these two species. {\textcopyright} 2017 The Author(s).",
keywords = "3' flanking region, 5' flanking region, Africa, Animals, Article, Bulinus, DNA sequence, DNA, Helminth, Disease Eradication, DraI, Humans, Hybridisation, Inter-repeat linkers, Middle East, Nucleic Acid Hybridization, Polymerase Chain Reaction, Repetitive Sequences, Nucleic Acid, Reverse line blot (RLB), Schistosoma, Schistosoma bovis, Schistosoma haematobium, Schistosomiasis haematobia, Species Specificity, adult, amplicon, animal, chemistry, classification, controlled study, disease eradication, gene amplification, genetics, genomic DNA, helminth DNA, human, hybridization, interspersed repeat, nonhuman, nucleic acid hybridization, nucleotide repeat, nucleotide sequence, parasite identification, parasitology, physiology, polymerase chain reaction, repetitive DNA, schistosomiasis haematobia, sequence alignment, sequence analysis, species difference, species differentiation, species identification",
author = "I. Abbasi and B.L. Webster and C.H. King and D. Rollinson and J. Hamburger",
note = "Cited By :8 Export Date: 30 January 2023 Correspondence Address: Hamburger, J.; Department of Microbiology and Molecular Genetics, Israel; email: joseph.hamburger@mail.huji.ac.il Chemicals/CAS: DNA, Helminth Funding details: National Institute of Allergy and Infectious Diseases, NIAID, R21AI076672 Funding details: Fogarty International Center, FIC, R01TW008067 Funding details: National Health Research Institutes, NHRI Funding text 1: This study was supported by National Institutes of Health Research Grants R01TW008067 and R21AI076672 funded by the Fogarty International Center and the National Institute of Allergy and Infectious Diseases. 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year = "2017",
doi = "https://doi.org/10.1186/s13071-017-2281-7",
language = "الإنجليزيّة",
volume = "10",
journal = "Parasites and Vectors",
issn = "1756-3305",
publisher = "BioMed Central Ltd.",
number = "1",
}
