The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms

Alona Keren-Paz; Harsh Maan; Iris Karunker; Tsviya Olender; Sergey Kapishnikov; Simon Dersch; Elena Kartvelishvily; Sharon G. Wolf; Assaf Gal; Peter L. Graumann; Ilana Kolodkin-Gal

doi:10.1016/j.isci.2022.104308

The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms

Alona Keren-Paz, Harsh Maan, Iris Karunker, Tsviya Olender, Sergey Kapishnikov, Simon Dersch, Elena Kartvelishvily, Sharon G. Wolf, Assaf Gal, Peter L. Graumann, Ilana Kolodkin-Gal

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

In nature, bacteria reside in biofilms– multicellular differentiated communities held together by an extracellular matrix. This work identified a novel subpopulation—mineral-forming cells—that is essential for biofilm formation in Bacillus subtilis biofilms. This subpopulation contains an intracellular calcium-accumulating niche, in which the formation of a calcium carbonate mineral is initiated. As the biofilm colony develops, this mineral grows in a controlled manner, forming a functional macrostructure that serves the entire community. Consistently, biofilm development is prevented by the inhibition of calcium uptake. Our results provide a clear demonstration of the orchestrated production of calcite exoskeleton, critical to morphogenesis in simple prokaryotes.

Original language	English
Article number	104308
Journal	iScience
Volume	25
Issue number	6
Early online date	27 Apr 2022
DOIs	https://doi.org/10.1016/j.isci.2022.104308
State	Published - 17 Jun 2022

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title = "The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms",

abstract = "In nature, bacteria reside in biofilms– multicellular differentiated communities held together by an extracellular matrix. This work identified a novel subpopulation—mineral-forming cells—that is essential for biofilm formation in Bacillus subtilis biofilms. This subpopulation contains an intracellular calcium-accumulating niche, in which the formation of a calcium carbonate mineral is initiated. As the biofilm colony develops, this mineral grows in a controlled manner, forming a functional macrostructure that serves the entire community. Consistently, biofilm development is prevented by the inhibition of calcium uptake. Our results provide a clear demonstration of the orchestrated production of calcite exoskeleton, critical to morphogenesis in simple prokaryotes.",

author = "Alona Keren-Paz and Harsh Maan and Iris Karunker and Tsviya Olender and Sergey Kapishnikov and Simon Dersch and Elena Kartvelishvily and Wolf, {Sharon G.} and Assaf Gal and Graumann, {Peter L.} and Ilana Kolodkin-Gal",

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AU - Keren-Paz, Alona

AU - Maan, Harsh

AU - Karunker, Iris

AU - Olender, Tsviya

AU - Kapishnikov, Sergey

AU - Dersch, Simon

AU - Kartvelishvily, Elena

AU - Wolf, Sharon G.

AU - Gal, Assaf

AU - Graumann, Peter L.

AU - Kolodkin-Gal, Ilana

PY - 2022/6/17

Y1 - 2022/6/17

N2 - In nature, bacteria reside in biofilms– multicellular differentiated communities held together by an extracellular matrix. This work identified a novel subpopulation—mineral-forming cells—that is essential for biofilm formation in Bacillus subtilis biofilms. This subpopulation contains an intracellular calcium-accumulating niche, in which the formation of a calcium carbonate mineral is initiated. As the biofilm colony develops, this mineral grows in a controlled manner, forming a functional macrostructure that serves the entire community. Consistently, biofilm development is prevented by the inhibition of calcium uptake. Our results provide a clear demonstration of the orchestrated production of calcite exoskeleton, critical to morphogenesis in simple prokaryotes.

AB - In nature, bacteria reside in biofilms– multicellular differentiated communities held together by an extracellular matrix. This work identified a novel subpopulation—mineral-forming cells—that is essential for biofilm formation in Bacillus subtilis biofilms. This subpopulation contains an intracellular calcium-accumulating niche, in which the formation of a calcium carbonate mineral is initiated. As the biofilm colony develops, this mineral grows in a controlled manner, forming a functional macrostructure that serves the entire community. Consistently, biofilm development is prevented by the inhibition of calcium uptake. Our results provide a clear demonstration of the orchestrated production of calcite exoskeleton, critical to morphogenesis in simple prokaryotes.

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DO - 10.1016/j.isci.2022.104308

M3 - مقالة

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ER -

The roles of intracellular and extracellular calcium in Bacillus subtilis biofilms

Abstract

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