@inbook{9ab72a4df0f3495e8bafc3fed7f0bf6b,
title = "The Ras Recruitment System (RRS) for the identification and characterization of protein–protein interactions",
abstract = "Protein–protein interactions are the basis for all biochemical cellular activities. The Ras Recruitment System, RRS, is a method for studying interactions between known proteins as well as identification of novel interactions following a cDNA library screen. The method is based on the recruitment of the Ras protein to the plasma membrane via protein–protein interactions. The interaction between proteins is studied in a temperature-sensitive yeast Saccharomyces cerevisiae mutant strain. This mutant is able to grow under restrictive temperature conditions when the Ras viability pathway becomes activated as a result of a positive protein–protein interaction. The RRS complements the limitations and problems that arise from the yeast two-hybrid system.",
keywords = "Cytosol, Inducible promoters, Plasma membrane, Protein recruitment, Protein–protein interaction, Ras, Temperature sensitive, Two-hybrid system",
author = "Ami Aronheim",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018.",
year = "2018",
doi = "10.1007/978-1-4939-7871-7_5",
language = "الإنجليزيّة",
series = "Methods in Molecular Biology",
pages = "61--73",
booktitle = "Methods in Molecular Biology",
}