The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells

Evelyne Zeira; Rinat Abramovitch; Karen Meir; Sharona Even Ram; Yaniv Gil; Baruch Bulvik; Zohar Bromberg; Or Levkovitch; Nathalie Nahmansson; Revital Adar; Benjamin Reubinoff; Eithan Galun; Michal Gropp

doi:10.18632/oncotarget.5787

The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells

Evelyne Zeira
, Rinat Abramovitch
, Karen Meir
, Sharona Even Ram
, Yaniv Gil
, Baruch Bulvik
, Zohar Bromberg
, Or Levkovitch
, Nathalie Nahmansson
, Revital Adar
, Benjamin Reubinoff
, Eithan Galun
, Michal Gropp

Research output: Contribution to journal › Article › peer-review

Abstract

The function of imprinted H19 long non-coding RNA is still controversial. It is highly expressed in early embryogenesis and decreases after birth and re-expressed in cancer. To study the role of H19 in oncogenesis and pluripotency, we down-regulated H19 expression in vitro and in vivo in pluripotent human embryonic carcinoma (hEC) and embryonic stem (hES) cells. H19 knockdown resulted in a decrease in the expression of the pluripotency markers Oct4, Nanog, TRA-1-60 and TRA-1-81, and in the up-regulation of SSEA1; it further attenuated cell proliferation, decreased cell-matrix attachment, and up-regulated E-Cadherin expression. SCID-Beige mice transplanted with H19 down-regulated hEC cells exhibited slower kinetics of tumor formation, resulting in an increased animal survival. Tumors derived from H19 down-regulated cells showed a decrease in the expression of pluripotency markers and up-regulation of SSEA-1 and E-cadherin. Our results suggest that H19 oncogenicity in hEC cells is mediated through the regulation of the pluripotency state.

Original language	English
Pages (from-to)	34691-34703
Number of pages	13
Journal	Oncotarget
Volume	6
Issue number	33
DOIs	https://doi.org/10.18632/oncotarget.5787
State	Published - 2015
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

H19lncRNA
HEC cells
Oncogenesis
Pluripotency

ASJC Scopus subject areas

Oncology

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10.18632/oncotarget.5787

Cite this

Zeira, E., Abramovitch, R., Meir, K., Ram, S. E., Gil, Y., Bulvik, B., Bromberg, Z., Levkovitch, O., Nahmansson, N., Adar, R., Reubinoff, B., Galun, E., & Gropp, M. (2015). The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells. Oncotarget, 6(33), 34691-34703. https://doi.org/10.18632/oncotarget.5787

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title = "The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells",

abstract = "The function of imprinted H19 long non-coding RNA is still controversial. It is highly expressed in early embryogenesis and decreases after birth and re-expressed in cancer. To study the role of H19 in oncogenesis and pluripotency, we down-regulated H19 expression in vitro and in vivo in pluripotent human embryonic carcinoma (hEC) and embryonic stem (hES) cells. H19 knockdown resulted in a decrease in the expression of the pluripotency markers Oct4, Nanog, TRA-1-60 and TRA-1-81, and in the up-regulation of SSEA1; it further attenuated cell proliferation, decreased cell-matrix attachment, and up-regulated E-Cadherin expression. SCID-Beige mice transplanted with H19 down-regulated hEC cells exhibited slower kinetics of tumor formation, resulting in an increased animal survival. Tumors derived from H19 down-regulated cells showed a decrease in the expression of pluripotency markers and up-regulation of SSEA-1 and E-cadherin. Our results suggest that H19 oncogenicity in hEC cells is mediated through the regulation of the pluripotency state.",

keywords = "H19lncRNA, HEC cells, Oncogenesis, Pluripotency",

author = "Evelyne Zeira and Rinat Abramovitch and Karen Meir and Ram, \{Sharona Even\} and Yaniv Gil and Baruch Bulvik and Zohar Bromberg and Or Levkovitch and Nathalie Nahmansson and Revital Adar and Benjamin Reubinoff and Eithan Galun and Michal Gropp",

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doi = "10.18632/oncotarget.5787",

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T1 - The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells

AU - Zeira, Evelyne

AU - Abramovitch, Rinat

AU - Meir, Karen

AU - Ram, Sharona Even

AU - Gil, Yaniv

AU - Bulvik, Baruch

AU - Bromberg, Zohar

AU - Levkovitch, Or

AU - Nahmansson, Nathalie

AU - Adar, Revital

AU - Reubinoff, Benjamin

AU - Galun, Eithan

AU - Gropp, Michal

PY - 2015

Y1 - 2015

N2 - The function of imprinted H19 long non-coding RNA is still controversial. It is highly expressed in early embryogenesis and decreases after birth and re-expressed in cancer. To study the role of H19 in oncogenesis and pluripotency, we down-regulated H19 expression in vitro and in vivo in pluripotent human embryonic carcinoma (hEC) and embryonic stem (hES) cells. H19 knockdown resulted in a decrease in the expression of the pluripotency markers Oct4, Nanog, TRA-1-60 and TRA-1-81, and in the up-regulation of SSEA1; it further attenuated cell proliferation, decreased cell-matrix attachment, and up-regulated E-Cadherin expression. SCID-Beige mice transplanted with H19 down-regulated hEC cells exhibited slower kinetics of tumor formation, resulting in an increased animal survival. Tumors derived from H19 down-regulated cells showed a decrease in the expression of pluripotency markers and up-regulation of SSEA-1 and E-cadherin. Our results suggest that H19 oncogenicity in hEC cells is mediated through the regulation of the pluripotency state.

AB - The function of imprinted H19 long non-coding RNA is still controversial. It is highly expressed in early embryogenesis and decreases after birth and re-expressed in cancer. To study the role of H19 in oncogenesis and pluripotency, we down-regulated H19 expression in vitro and in vivo in pluripotent human embryonic carcinoma (hEC) and embryonic stem (hES) cells. H19 knockdown resulted in a decrease in the expression of the pluripotency markers Oct4, Nanog, TRA-1-60 and TRA-1-81, and in the up-regulation of SSEA1; it further attenuated cell proliferation, decreased cell-matrix attachment, and up-regulated E-Cadherin expression. SCID-Beige mice transplanted with H19 down-regulated hEC cells exhibited slower kinetics of tumor formation, resulting in an increased animal survival. Tumors derived from H19 down-regulated cells showed a decrease in the expression of pluripotency markers and up-regulation of SSEA-1 and E-cadherin. Our results suggest that H19 oncogenicity in hEC cells is mediated through the regulation of the pluripotency state.

KW - H19lncRNA

KW - HEC cells

KW - Oncogenesis

KW - Pluripotency

UR - https://www.scopus.com/pages/publications/84946605500

U2 - 10.18632/oncotarget.5787

DO - 10.18632/oncotarget.5787

M3 - Article

C2 - 26415227

SN - 1949-2553

VL - 6

SP - 34691

EP - 34703

JO - Oncotarget

JF - Oncotarget

IS - 33

ER -

The knockdown of H19lncRNA reveals its regulatory role in pluripotency and tumorigenesis of human embryonic carcinoma cells

Abstract

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Keywords

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