TY - JOUR
T1 - The isocitrate dehydrogenase 1 gene is associated with the climacteric response in tomato fruit ripening
AU - Gamrasni, Dan
AU - Erov, Maayan
AU - Saar, Lotem
AU - Raz, Amir
AU - Glikman, Michal
AU - Sonawane, Prashant D.
AU - Aharoni, Asaph
AU - Goldway, Martin
N1 - CRediT authorship contribution statement: Dan Gamrasni: Conceptualization, Writing - original draft, Supervision. Maayan Erov: Investigation. Lotem Saar: Investigation. Amir Raz: Formal analysis. Michal Glikman: Formal analysis. Prashant D. Sonawane: Resources. Asaph Aharoni: Conceptualization, Resources, Writing - review & editing. Martin Goldway: Conceptualization, Writing - original draft, Supervision.
PY - 2020/8
Y1 - 2020/8
N2 - Climacteric ripening is related to a respiratory climax accompanied by an exponential increase in ethylene production. This association is not fully understood. Tomato fruit were exposed to the ethylene antagonist 1-methylcyclopropene (1-MCP) at different ripening stages and their transcriptome was monitored. ISOCITRATE DEHYDROGENASE 1 (ICDH, Solyc01g005560.2.1) was the only respiratory process related gene that was down regulated at all ripening stages including the mature green. Silencing SlICDH1using VIGS (virus indused gene silencing), to about 50 % of its native expression, in Micro-Tom tomatoes, reduced both respiration and ethylene production of the harvested fruit. Silenced SlICDH1 tomatoes also exhibited prolonged ripening and reduced susceptibility to the pathogen Botrytis cinerea. In addition, ethylene response factors binding sites were identified in the promotor and in the first intron of SlICDH1. These results suggest that regulation of SlICDH1 is a key step in the initiation of the climacteric rise of respiration via ethylene regulation.
AB - Climacteric ripening is related to a respiratory climax accompanied by an exponential increase in ethylene production. This association is not fully understood. Tomato fruit were exposed to the ethylene antagonist 1-methylcyclopropene (1-MCP) at different ripening stages and their transcriptome was monitored. ISOCITRATE DEHYDROGENASE 1 (ICDH, Solyc01g005560.2.1) was the only respiratory process related gene that was down regulated at all ripening stages including the mature green. Silencing SlICDH1using VIGS (virus indused gene silencing), to about 50 % of its native expression, in Micro-Tom tomatoes, reduced both respiration and ethylene production of the harvested fruit. Silenced SlICDH1 tomatoes also exhibited prolonged ripening and reduced susceptibility to the pathogen Botrytis cinerea. In addition, ethylene response factors binding sites were identified in the promotor and in the first intron of SlICDH1. These results suggest that regulation of SlICDH1 is a key step in the initiation of the climacteric rise of respiration via ethylene regulation.
UR - http://www.scopus.com/inward/record.url?scp=85083695791&partnerID=8YFLogxK
U2 - 10.1016/j.postharvbio.2020.111219
DO - 10.1016/j.postharvbio.2020.111219
M3 - مقالة
SN - 0925-5214
VL - 166
JO - Postharvest Biology and Technology
JF - Postharvest Biology and Technology
M1 - 111219
ER -