The function of EHD2 in endocytosis and defense signaling is affected by SUMO

Maya Bar, Silvia Schuster, Meirav Leibman, Ran Ezer, Adi Avni

Research output: Contribution to journalArticlepeer-review


Post-translational modification of target proteins by the small ubiquitin-like modifier protein (SUMO) regulates many cellular processes. SUMOylation has been shown to regulate cellular localization and function of a variety of proteins, in some cases affecting nuclear import or export. We have previously characterized two EHDs (EH domain containing proteins) in Arabidospis and showed their involvement in plant endocytosis. AtEHD2 has an inhibitory effect on endocytosis of transferrin, FM-4-64, and the leucine rich repeat receptor like protein LeEix2, an effect that requires and intact coiled-coil domain. Inhibition of endocytosis of LeEix2 by EHD2 is effective in inhibiting defense responses mediated by the LeEix2 receptor in response to its ligand EIX. In the present work we demonstrate that SUMOylation of EHD2 appears to be required for EHD2-induced inhibition of LeEix2 endocytosis. Indeed, we found that a mutant form of EHD2, possessing a defective SUMOylation site, has an increased nuclear abundance, can no longer be SUMOylated and is no longer effective in inhibiting LeEix2 endocytosis or defense signaling in response to EIX.

Original languageEnglish
Pages (from-to)509-518
Number of pages10
JournalPlant Molecular Biology
Issue number4-5
StatePublished - Mar 2014


  • EH domain
  • EHD2
  • EIX
  • Endocytosis
  • LeEix
  • SUMO
  • SUMOylation

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science


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