TY - JOUR
T1 - The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem Cells
AU - Rufaihah, Abdul Jalil
AU - Cheyyatraivendran, Suganya
AU - Mazlan, Muhammad Danial Mohd
AU - Lim, Kenrich
AU - Chong, Mark Seow Khoon
AU - Mattar, Citra Nurfarah Zaini
AU - Chan, Jerry Kok Yen
AU - Kofidis, Theodoros
AU - Seliktar, Dror
N1 - Publisher Copyright: © Copyright © 2018 Rufaihah, Cheyyatraivendran, Mazlan, Lim, Chong, Mattar, Chan, Kofidis and Seliktar.
PY - 2018/12/21
Y1 - 2018/12/21
N2 - Hydrogel materials have been successfully used as matrices to explore the role of biophysical and biochemical stimuli in directing stem cell behavior. Here, we present our findings on the role of modulus in guiding bone marrow fetal mesenchymal stem cell (BMfMSC) fate determination using semi-synthetic hydrogels made from PEG-fibrinogen (PF). The BMfMSCs were cultivated in the PF for up to 2 weeks to study the influence of matrix modulus (i.e., cross-linking density of the PF) on BMfMSC survival, morphology and integrin expression. Both two-dimensional (2D) and three-dimensional (3D) culture conditions were employed to examine the BMfMSCs as single cells or as cell spheroids. The hydrogel modulus affected the rate of BMfMSC metabolic activity, the integrin expression levels and the cell morphology, both as single cells and as spheroids. The cell seeding density was also found to be an important parameter of the system in that high densities were favorable in facilitating more cell-to-cell contacts that favored higher metabolic activity. Our findings provide important insight about design of a hydrogel scaffold that can be used to optimize the biological response of BMfMSCs for various tissue engineering applications.
AB - Hydrogel materials have been successfully used as matrices to explore the role of biophysical and biochemical stimuli in directing stem cell behavior. Here, we present our findings on the role of modulus in guiding bone marrow fetal mesenchymal stem cell (BMfMSC) fate determination using semi-synthetic hydrogels made from PEG-fibrinogen (PF). The BMfMSCs were cultivated in the PF for up to 2 weeks to study the influence of matrix modulus (i.e., cross-linking density of the PF) on BMfMSC survival, morphology and integrin expression. Both two-dimensional (2D) and three-dimensional (3D) culture conditions were employed to examine the BMfMSCs as single cells or as cell spheroids. The hydrogel modulus affected the rate of BMfMSC metabolic activity, the integrin expression levels and the cell morphology, both as single cells and as spheroids. The cell seeding density was also found to be an important parameter of the system in that high densities were favorable in facilitating more cell-to-cell contacts that favored higher metabolic activity. Our findings provide important insight about design of a hydrogel scaffold that can be used to optimize the biological response of BMfMSCs for various tissue engineering applications.
KW - PEGylated fibrinogen
KW - biomaterials
KW - hydrogel
KW - matrix stiffness
KW - scaffold
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85063459946&partnerID=8YFLogxK
U2 - https://doi.org/10.3389/fphys.2018.01555
DO - https://doi.org/10.3389/fphys.2018.01555
M3 - مقالة
SN - 1664-042X
VL - 9
JO - Frontiers in Physiology
JF - Frontiers in Physiology
M1 - 1555
ER -