The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosis coprodetection

H. Salant, I. Abbasi, J. Hamburger

Research output: Contribution to journalArticlepeer-review


We have previously developed a polymerase chain reaction (PCR) assay for detection of Echinococcus granulosus infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of E. granulosus for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for E. granulosus with no cross-amplification of DNA from closely related helminths, including Echinococcus multilocularis. Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for premortem identification of E. granulosus-infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.
Original languageEnglish
Pages (from-to)883-887
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number5
StatePublished - 2012


  • Animals
  • Base Sequence
  • Canis familiaris
  • DNA
  • DNA Primers
  • DNA polymerase
  • DNA, Protozoan
  • Echinococcus granulosus
  • Echinococcus multilocularis
  • Feces
  • Humans
  • Nucleic Acid Amplification Techniques
  • Parasite Egg Count
  • RNA 12S
  • Sensitivity and Specificity
  • Vermes
  • agar gel electrophoresis
  • amplicon
  • animal experiment
  • animal model
  • article
  • autopsy
  • copro polymerase chain reaction
  • diagnostic kit
  • diagnostic test accuracy study
  • dog disease
  • echinococcosis
  • egg
  • endemic disease
  • feces analysis
  • gene amplification
  • genome
  • gold standard
  • loop mediated isothermal amplification
  • nonhuman
  • parasite antigen
  • parasite identification
  • polymerase chain reaction
  • sensitivity and specificity
  • species differentiation
  • spectrophotometry
  • tandem repeat


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