The delicate relationship between DNA replication timing and gene expression

Avraham Greenberg, Britny Blumenfeld, Itamar Simon

Research output: Contribution to journalReview articlepeer-review


The order in which different domains of DNA undergo replication forms a highly regulated, precise pattern. Replication timing (RT) has been studied at a variety of resolutions and can now be mapped in a genome-wide fashion. RT is correlated with a plethora of genetic and epigenetic features, and here, we highlight recent developments in our understanding of the relationship between RT and transcription. In spite of the general correlation between early replication and transcription, recent single-cell experiments and detailed time-course models indicate that the correlation is not absolute. Moreover, asynchronous replicating regions are associated with monoallelic expressed genes, but the active allele is not always the early one. We propose that RT contributes to transcription at a few levels but that it is not a prerequisite for it.

Original languageAmerican English
Pages (from-to)8-15
Number of pages8
JournalCurrent Opinion in Systems Biology
StatePublished - Feb 2020


  • Asynchronous replication
  • Chromatin
  • DNA replication
  • Epigenetics
  • Gene expression
  • Monoallelic expression
  • Replication timing
  • Transcription

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Applied Mathematics
  • General Biochemistry,Genetics and Molecular Biology
  • Computer Science Applications
  • Modelling and Simulation


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