The amazing acrobat: Yeastʹs histone h3k56 juggles several important roles while maintaining perfect balance

Acetylation on lysine 56 of histone H3 of the yeast Saccharomyces cerevisiae has been impli-cated in many cellular processes that affect genome stability. Despite being the object of much re-search, the complete scope of the roles played by K56 acetylation is not fully understood even today. The acetylation is put in place at the S‐phase of the cell cycle, in order to flag newly synthesized histones that are incorporated during DNA replication. The signal is removed by two redundant deacetylases, Hst3 and Hst4, at the entry to G2/M phase. Its crucial location, at the entry and exit points of the DNA into and out of the nucleosome, makes this a central modification, and dictates that if acetylation and deacetylation are not well concerted and executed in a timely fashion, severe genomic instability arises. In this review, we explore the wealth of information available on the many roles played by H3K56 acetylation and the deacetylases Hst3 and Hst4 in DNA replication and repair.