@article{7d3dcab096514c3a9042635b0fd5eca4,
title = "Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria",
abstract = "Riboswitches and attenuators are cis-regulatory RNA elements, most of which control bacterial gene expression via metabolite-mediated, premature transcription termination. We developed an unbiased experimental approach for genome-wide discovery of such ribo-regulators in bacteria. We also devised an experimental platform that quantitatively measures the in vivo activity of all such regulators in parallel and enables rapid screening for ribo-regulators that respond to metabolites of choice. Using this approach, we detected numerous antibioticresponsive ribo-regulators that control antibiotic resistance genes in pathogens and in the human microbiome. Studying one such regulator in Listeria monocytogenes revealed an attenuation mechanism mediated by antibiotic-stalled ribosomes. Our results expose broad roles for conditional termination in regulating antibiotic resistance and provide a tool for discovering riboswitches and attenuators that respond to previously unknown ligands.",
author = "Daniel Dar and Maya Shamir and Mellin, {J. R.} and Mikael Koutero and Noam Stern-Ginossar and Pascale Cossart and Rotem Sorek",
note = "We thank O. Wurtzel, I. Karunker, S. Doron, G. Amitai, G. Ofir, A. Millman, Y. Voichek, S. Melamed, and R. Nir-Paz for insightful discussion. R.S. was supported, in part, by the Israel Science Foundation (personal grant 1303/12 and I-CORE grant 1796/12), the European Research Council (ERC)–Starting Grants program (grant 260432), Human Frontier Science Program (grant RGP0011/2013 to R.S. and P.C), the Abisch-Frenkel foundation, the Pasteur-Weizmann council grant (to R.S and P.C), the Minerva Foundation, the Leona M. and Harry B. Helmsley Charitable Trust, and by a Deutsch-Israelische Projektkooperation grant from the Deutsche Forschungsgemeinschaft. P.C was supported by an ERC–Advanced Grants grant (BacCellEpi, #670823). Reference IRB for the microbiome analysis is 0315-15-HMO. Sequencing data was deposited in the European Nucleotide Database (ENA), accession no. PRJEB12568. D.D. and R.S. are inventors on U.S. provisional patent application 62/200,662.",
year = "2016",
month = apr,
doi = "https://doi.org/10.1126/science.aad9822",
language = "الإنجليزيّة",
volume = "352",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6282",
}