T-plastin is essential for basement membrane assembly and epidermal morphogenesis

Eyal Dor-On, Shaul Raviv, Yonatan Cohen, Orit Adir, Krishnanand Padmanabhan, Chen Luxenburg

Research output: Contribution to journalArticlepeer-review


The establishment of epithelial architecture is a complex process involving cross-talk between cells and the basement membrane. Basement membrane assembly requires integrin activity but the role of the associated actomyosin cytoskeleton is poorly understood. Here, we identify the actin-bundling protein T-plastin (Pls3) as a regulator of basement membrane assembly and epidermal morphogenesis. In utero depletion of Pls3 transcripts in mouse embryos caused basement membrane and polarity defects in the epidermis but had little effect on cell adhesion and differentiation. Loss-of-function experiments demonstrated that the apicobasal polarity defects were secondary to the disruption of the basementmembrane. However, the basement membrane itself was profoundly sensitive to subtle perturbations in the actin cytoskeleton.We further show that Pls3 localized to the cell cortex, where it was essential for the localization and activation of myosin II. Inhibition of myosin II motor activity disrupted basement membrane organization. Our results provide insights into the regulation of cortical actomyosin and its importance for basement membrane assembly and skin morphogenesis.

Original languageEnglish
Article numberaal3154
JournalScience Signaling
Issue number481
StatePublished - 30 May 2017

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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